张坤,张恒,于志明,陈锐博,郑振宇,李春丽

• 1:河南农业大学牧医工程学院

摘要(Abstract)：

以大肠杆菌的6个管家基因(recA,aspC,ldh,gapdh,mdh,16S rRNA)作为候选的内参基因,大肠杆菌在不同浓度猪β防御素2(0、37.5、75、150 mg·L-1)的胁迫下培养不同时间(1 h和4 h)后,应用实时荧光定量PCR(RT-qPCR)技术比较这6个内参基因表达的稳定性,并通过GeNorm软件分析。结果表明,大肠杆菌中recA基因表达最稳定,其次是aspC和gapdh。并且16S rRNA的相对表达量最高,而ldh的表达量最低。

关键词(KeyWords)： 猪β防御素2;实时荧光定量PCR;大肠杆菌;内参基因

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31101694)

作者(Author): 张坤,张恒,于志明,陈锐博,郑振宇,李春丽

DOI: 10.16445/j.cnki.1000-2340.2016.01.012

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