任学良,李立芹,许力,郭玉双,鲁黎明

• 1:贵州省烟草科学研究院烟草行业分子遗传重点实验室
• 2:四川农业大学农学院

摘要(Abstract)：

为研究烟草糖基转移酶的生物学功能,根据在GenBank上登录的烟草糖基转移酶基因NtGT5b(登录号BAD93690.1)的CDS序列,设计特异性引物,并以烟草栽培品种K 326总RNA逆转录后的c DNA为模板,采用PCR方法,成功获得了烟草糖基转移酶基因NtGT5b的全长CDS序列。生物信息学分析结果表明,该基因CDS全长1 458 bp,所编码的蛋白质包含485个氨基酸。其相对分子质量为54.31 k D,等电点为5.47。在二级结构上,α-螺旋和无规则卷曲为NtGT5b蛋白质的主要结构形式,而β-折叠延伸链与β-转角则相对较少。在三级结构上,该蛋白质主要由α-螺旋与β-折叠构成。NtGT5b蛋白质是一个亲水性蛋白质,含有3个糖基化位点,不含信号肽,包含有糖基转移酶家族的功能保守域PLN02410,属于GTB类型的糖基转移酶超级家族,推测具有糖基转移的功能。

关键词(KeyWords)： 烟草;糖基转移酶;基因

Abstract:

Keywords:

基金项目(Foundation): 国家烟草专卖局科技项目(110201401007(JY-07));; 贵州省烟草科学研究院科技项目(烟草基因遗传转化及表型分析)

作者(Author): 任学良,李立芹,许力,郭玉双,鲁黎明

DOI: 10.16445/j.cnki.1000-2340.2016.04.002

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