张卫辉,朱秋凤,张永静,黄强

• 1:中国人民大学统计学院
• 2:中国农业大学动物科技学院
• 3:禾丰食品股份有限公司

摘要(Abstract)：

【目的】通过分析不同含量槲皮素对仔猪生长性能、肠道抗氧化指标、炎症细胞因子分泌及紧密连接蛋白基因表达的影响，探究槲皮素在改善仔猪应激综合征的作用。【方法】选取216头健康、初始体质量为(9.5±0.5)kg(杜×长×大)仔猪，随机分为3组(对照组、试验1组和试验2组),分别饲喂基础日粮、基础日粮+200 mg·kg~(-1)槲皮素日粮和基础日粮+400 mg·kg~(-1)槲皮素日粮，每组6个重复，每个重复12头仔猪(公、母各6头),试验期为15 d。【结果】1)与对照组相比，试验1组和试验2组均提高了仔猪平均日增重，显著降低了腹泻率。2)与对照组相比，试验1组和试验2组显著提高了仔猪空肠黏膜谷胱甘肽过氧化物酶(glutathione peroxidase, GPx)、超氧化物歧化酶(superoxide dismutase, SOD)和过氧化氢酶(catalase, CAT)活性，显著降低脂质过氧化物丙二醛(malondialdehyde, MDA)含量。其中，GPx、SOD和CAT活性随着槲皮素添加含量提高而显著增加。3)与对照组相比，试验1组和试验2组显著降低了空肠黏膜中白细胞介素2(interleukin-2,IL-2)、白细胞介素1β(interleukin-1β,IL-1β)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)水平，显著提高了白细胞介素10(interleukin-10,IL-10)水平；与其他组相比，试验2组分泌型免疫球蛋白A(secretory immunoglobulin A,sIgA)水平显著增加。4)与对照组相比，试验1组和试验2组显著提高了仔猪空肠黏膜中紧密连接蛋白基因Claudin-1、Occludin和ZO-2mRNA相对表达量，并且试验2组显著提高了ZO-1 mRNA相对表达量；与试验1组相比，试验2组显著提高仔猪空肠黏膜中紧密连接蛋白基因Claudin-1和Occludin mRNA相对表达量。【结论】日粮添加槲皮素可以降低断奶后仔猪腹泻率，改善生长性能，增强肠道抗氧化功能，提高抗炎因子分泌，抑制促炎因子分泌，促进肠道紧密连接蛋白相关基因表达。

关键词(KeyWords)： 仔猪;槲皮素;生长性能;抗氧化功能;炎症细胞因子;紧密连接蛋白;基因表达

Abstract:

Keywords:

基金项目(Foundation): 国家生猪产业技术体系建设专用项(CARS-35);; 辽宁省科技重大专项(2019JH1/10200002)

作者(Author): 张卫辉,朱秋凤,张永静,黄强

DOI: 10.16445/j.cnki.1000-2340.20220510.001

参考文献(References)：

• [1] LI Y,YAO J Y,YANG C Y,et al.Quercetin,inflammation and immunity[J].Nutrients,2016,8(3):167-181.
• [2] 李阳，苏艳瑜，李国豪，等.槲皮素通过Nrf2通路对糖尿病大鼠胰腺氧化损伤的拮抗作用机制[J].食品科学，2021,42(5):208-214.LI Y,SU Y Y,LI G H,et al.Antagonistic effect of quercetin against oxidative pancreatic injury in diabetic rats via Nrf2 pathway[J].Food Science,2021,42(5):208-214.
• [3] 张红娜，周玉法，刘敬博，等.基于NF-κB和Nrf2信号通路探讨槲皮素的护肝功效及其作用机理[J].西北农业学报，2020,29(1):143-149.ZHANG H N,ZHOU Y F,LIU J B,et al.Exploring protective effects and mechanisms of quercetin on liver injury based on both NF-κB and Nrf2 signaling pathways[J].Acta Agriculturae Boreali-Occidentalis Sinica,2020,29(1):143-149.
• [4] WANG S G,YAO J Y,ZHOU B,et al.Bacteriostatic effect of quercetin as an antibiotic alternative in vivo and its antibacterial mechanism in vitro[J].Journal of Food Protection,2018,81(1):68-78.
• [5] ALíA M,MATEOS R,RAMOS S,et al.Influence of quercetin and rutin on growth and antioxidant defense system of a human hepatoma cell line (HepG2)[J].European Journal of Nutrition,2006,45(1):19-28.
• [6] WANG D F,ZHOU L L,ZHOU H L,et al.Effects of Piper sarmentosum extract on the growth performance,antioxidant capability and immune response in weaned piglets[J].Journal of Animal Physiology and Animal Nutrition,2017,101(1):105-112.
• [7] 刘明月.槲皮素抑制脂多糖诱导的RAW264.7细胞炎症反应机制及其与AMPK的关系[D].大连：辽宁师范大学，2018.LIU M Y.Quercetin inhibits the inflammatory response mechanism of lipopolysaccharide induced RAW264.7 cells and the relationship with AMPK[D].Dalian:Liaoning Normal University,2018.
• [8] 田瑞雪，孙耀宗，姚有昊，等.槲皮素对免疫低下小鼠免疫功能的影响[J].中国现代医药杂志，2019,21(9):13-16.TIAN R X,SUN Y Z,YAO Y H,et al.Effect of quercetin on immune function in immuno compromised mice[J].Modern Medicine Journal of China,2019,21(9):13-16.
• [9] PARMAR A B.Efficiency of dietary supplementation of flavonoid (quercetin),vegetable oil and its combination on growth traits and feed conversion in broilers[J].Journal of Animal Research,2020,10(4):557-562.
• [10] NRC.Nutrient Requirements of Swine [M].11th Edition.Washington DC:National Academy Press,2012:56.
• [11] HUANG Q,XU W,BAI K W,et al.Protective effects of leucine on redox status and mitochondrial-related gene abundance in the jejunum of intrauterine growth-retarded piglets during early weaning period[J].Archives of Animal Nutrition,2017,71(2):93-107.
• [12] 钟翔.Hsp70介导IUGR仔猪早期免疫功能损伤的机理及谷氨酰胺的营养调控研究[D].南京：南京农业大学，2010.ZHONG X.Study on the mechanisms of immune deficiency mediated by Hsp70in neonatal iugr piglet and nutrional regulation of glutamine[D].Nanjing:Nanjing Agricultural University,2010.
• [13] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J].Methods,2001,25(4):402-408.
• [14] KINACI M K,ERKASAP N,KUCUK A,et al.Effects of quercetin on apoptosis,NF-κB and NOS gene expression in renal ischemia/reperfusion injury[J].Experimental and Therapeutic Medicine,2012,3(2):249-254.
• [15] SUN L,XU G Q,DONG Y,et al.Quercetin protects against lipopolysaccharide-induced intestinal oxidative stress in broiler chickens through activation of Nrf2 pathway[J].Molecules,2020,25(5):1053.
• [16] ZOU Y,WEI H K,XIANG Q H,et al.Protective effect of quercetin on pig intestinal integrity after transport stress is associated with regulation oxidative status and inflammation[J].The Journal of Veterinary Medical Science,2016,78(9):1487-1494.
• [17] DOGAN Z,ELBE H,TASLIDERE E,et al.Effects of ciprofloxacin on fetal rat liver during pregnancy and protective effects of quercetin[J].Biotechnic & Histoche-mistry:Official Publication of the Biological Stain Commission,2017,92(7):481-486.
• [18] WU M,LU S J,ZHONG J H,et al.Protective effects of pterostilbene against myocardial ischemia/reperfusion injury in rats[J].Inflammation,2017,40(2):578-588.
• [19] 李泳欣.Nrf2-ARE信号通路抵御小鼠乳腺氧化应激的分子机制及槲皮素对其调控研究[D].杭州：浙江大学，2021.LI Y X.The molecular mechanisms of Nrf2-ARE signaling pathway against oxidative stress and its regulation by quercetin in mammary gland of mice[D].Hangzhou:Zhejiang University,2021.
• [20] 梅华迪，李袁飞，田琦，等.槲皮素对断奶仔猪生长性能、营养物质表观消化率、血清生化指标、粪便微生物及其代谢产物的影响[J].动物营养学报，2022,34(5):2884-2895.MEI H D,LI Y F,TIAN Q,et al.Effects of quercetin on growth performance,nutrient apparent digestibility,serum biochemical indices,fecal microorganisms and their metabolites of weaned piglets[J].Chinese Journal of Animal Nutrition,2022,34(5):2884-2895.
• [21] 王盛楠.槲皮素对AA肉鸡蛋白质消化利用的作用及机制[D].哈尔滨：东北农业大学，2018.WANG S N.Effects and mechanism of quercetin on protein digestion and utilization in AA broilers[D].Harbin:Northeast Agricultural University,2018.
• [22] 滕楠.槲皮素抑菌作用的体内和体外研究[D].哈尔滨：东北农业大学，2015.TENG N.Bacteriostasis of quercetin in vivo and in vitro[D].Harbin:Northeast Agricultural University,2015.
• [23] 蔡智谋，王晓慧，蒋冰之，等.槲皮素免疫学作用研究进展[J].教育教学论坛，2016(16):83-84.CAI Z M,WANG X H,JIANG B Z,et al.Research progress on the immunological effect of quercetin[J].Education Teaching Forum,2016(16):83-84.
• [24] SMITH F,CLARK J E,OVERMAN B L,et al.Early weaning stress impairs development of mucosal barrier function in the porcine intestine[J].American Journal of Physiology Gastrointestinal and Liver Physiology,2010,298(3):352-363.
• [25] HU C H,XIAO K,LUAN Z S,et al.Early weaning increases intestinal permeability,alters expression of cytokine and tight junction proteins,and activates mitogen-activated protein kinases in pigs[J].Journal of Animal Science,2013,91(3):1094-1101.
• [26] SUZUKI T,HARA H.Quercetin enhances intestinal barrier function through the assembly of zonnula occludens-2,occludin,and claudin-1 and the expression of claudin-4 in caco-2 cells[J].The Journal of Nutrition,2009,139(5):965-974.