云南4处酸性热泉中的变形菌门细菌多样性The diversities of Proteobacteria in four acidic hot springs in Yunnan
宋兆齐,王莉,刘秀花,梁峰
摘要(Abstract):
选择云南腾冲和龙陵地区的4处酸性热泉(p H:2.3~6.0,温度:47~96℃),通过Barcoded pyrosequencing技术及统计学分析,详细阐述了这些样点中变形菌门的物种和遗传多样性。本研究共获得了2 489条变形菌门16S rRNA基因序列,可划分为234个可操作分类单元(OTUs)。分类结果显示,热泉内涵盖了大量已知的目、科和属。而41%的OTUs可能代表了变形菌门的未知物种。系统发育分析显示,样点中存在着若干全新遗传类群,这些样点和美国黄石地区的热泉各自呈现不同的变形菌门遗传多样性。此外,本次调查的热泉彼此间存在着变形菌门群落结构的显著差异。
关键词(KeyWords): 变形菌门;多样性;系统发育分析;酸性热泉;云南
基金项目(Foundation): 国家自然科学基金项目(31300430)
作者(Author): 宋兆齐,王莉,刘秀花,梁峰
DOI: 10.16445/j.cnki.1000-2340.2016.03.015
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