木瓜蛋白酶水解猪肠黏膜制备肝素水解条件的优化Optimization of hydrolysis conditions for the heparin preparation by Papain hydrolyzing pig intestinal mucosa with papain
张秋会,徐俊涛,李苗云,高晓平,赵改名
摘要(Abstract)：
本研究对木瓜蛋白酶水解猪小肠黏膜制备肝素的条件进行了优化,并进行了验证试验。结果表明,木瓜蛋白酶酶解条件为:温度60℃,时间3 h,料液比为1∶7(g·L-1),酶底物质量分数0.80%,p H值8.8,盐浓度0.3mol·L-1;验证试验证明该水解条件是可靠的。
关键词(KeyWords)： 肝素;木瓜蛋白酶;水解
基金项目(Foundation): 河南省科技攻关计划项目(142102210442)
作者(Author): 张秋会,徐俊涛,李苗云,高晓平,赵改名
DOI: 10.16445/j.cnki.1000-2340.2018.06.017
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