番茄疫霉根腐病菌拮抗细菌HP8-1的鉴定及生物防治潜力Identification and biocontrol potential of antagonist bacteria HP8-1 against Phytophthora root rot of tomato
王艺茹,潘培培,沈虎生,张林林,王润东,何梦菡,申顺善
摘要(Abstract):
【目的】探究HP8-1在番茄上的生物防治潜力,为番茄疫霉根腐病的生物防治提供优良生物防治资源。【方法】通过形态学观察、生理生化特性检测并结合16S rRNA序列分析鉴定HP8-1菌株,探讨其对植物病原菌的抑菌效果和生物学功能及在番茄根际的定殖能力,并通过盆栽试验测定其对番茄疫霉根腐病的防治效果和对番茄生长的影响。【结果】HP8-1为多粘类芽孢杆菌(Paenibacillus polymyxa),对多种植物病原菌具有抑菌效果,具有解磷、分解蛋白、分解纤维素、产嗜铁素和产吲哚乙酸等生物学功能;HP8-1在番茄根系和根际土壤具有稳定的定殖能力,尤其是在接种疫霉菌的条件下其定殖量趋于增加并显著高于未接种疫霉菌的条件;HP8-1处理对番茄疫霉根腐病具有明显的防治效果,防治效果达到57.41%;同时,HP8-1处理显著促进番茄植株生长、提高叶绿素含量和根系活力。【结论】HP8-1菌株稳定定殖在番茄根系和根际土壤,防治番茄疫霉根腐病,促进番茄生长,是对番茄疫霉根腐病具有生物防治潜力的资源。
关键词(KeyWords): 根际促生细菌;多粘类芽孢杆菌;番茄疫霉根腐病;根际定殖;生物防治
基金项目(Foundation): 河南省重点科技攻关资助项目(202102110218);; 国家重点研发计划项目(2017YFD0201100)
作者(Author): 王艺茹,潘培培,沈虎生,张林林,王润东,何梦菡,申顺善
DOI: 10.16445/j.cnki.1000-2340.20231019.003
参考文献(References):
- [1]谢燕青,陈慧,梁朝晖,等.番茄疫霉根腐病的识别与综合防治[J].长江蔬菜,2008(4):22-23.XIE Y Q,CHEN H,LIANG C H,et al.Identification and comprehensive control of Phytophthora root rot in tomato[J].Journal of Changjiang Vegetables,2008(4):22-23.
- [2]默书霞,李金堂,于学祥,等.番茄疫霉根腐病的识别及防治[J].中国蔬菜,2009(17):27.MO S X,LI J T,YU X X,et al.Identification and control of Phytophthora root rot in tomato[J].China Vegetables,2009(17):27.
- [3]杨营业,白富丽.番茄疫霉根腐病的发生规律与防治[J].农业技术与装备,2014(14):38-39.YANG Y Y,BAI F L.Occurrence and control of Phytophthora root rot of tomato[J].Agricultural Technology&Equipment,2014(14):38-39.
- [4]程欢欢,余水,姚伟伟,等.辣椒炭疽病生防芽孢杆菌的筛选及田间防效[J].河南农业大学学报,2019,53(4):568-573.CHENG H H,YU S,YAO W W,et al.Screening of antagonistic Bacillus strains and control effect against pepper anthracnose in field trial[J].Journal of Henan Agricultural University,2019,53(4):568-573.
- [5]张蒙蒙,梁俊阳,王清福,等.烟草黑胫病生防细菌的鉴定和防效测定[J].河南农业大学学报,2022,56(2):219-227.ZHANG M M,LIANG J Y,WANG Q F,et al.Identification and control efficiency of biocontrol bacteria against tobacco black shank[J].Journal of Henan Agricultural University,2022,56(2):219-227.
- [6]张涵,赵莹,仲荣荣,等.生防细菌TB17的筛选、鉴定及其对苦瓜枯萎病的生防作用[J].河南农业大学学报,2022,56(3):429-437.ZHANG H,ZHAO Y,ZHONG R,et al.Screening,identification of biocontrol bacteria TB17 and its biocontrol effect on Fusarium wilt of bitter gourd[J].Journal of Henan Agricultural University,2022,56(3):429-437.
- [7]王荣波,陈姝樽,肖小露,等.枯草芽胞杆菌BS193对辣椒疫霉的拮抗作用及其相关生防因子检测[J].农业生物技术学报,2022,30(4):772-782.WANG R B,CHEN S Z,XIAO X L,et al.Antagonistic activity and related biocontrol factors detection of Bacillus subtilis BS193 on Phytophthora capsici[J].Journal of Agricultural Biotechnology,2022,30(4):772-782.
- [8]沈海斌,王前程,陈捷,等.三株木霉对番茄枯萎病的防治效果和机理研究[J].植物生理学报,2023,59(5):965-976.SEHN H B,WANG Q C,CHEN J,et al.Efficacy and mechanism of three Trichoderma strains for control of tomato Fusarium wilt[J].Plant Physiology Journal,2023,59(5):965-976.
- [9]冯志珍,李金岭,陈太春,等.番茄疫霉根腐病拮抗细菌FC12-05的筛选、鉴定及其抑菌活性初探[J].西北农林科技大学学报(自然科学版),2012,40(4):107-114.FENG Z Z,LI J L,CHEN T C,et al.Screening,identification and antibacterial activity of antagonistic bacteria FC12-05 against Phytophthora root rot of tomato[J].Journal of Northwest A&F University (Natural Science Edition),2012,40(4):107-114.
- [10]谢学文,赵昱榕,孙雪莹,等.番茄疫霉根腐病生防菌的分离鉴定及其防治效果[J].植物病理学报,2020,50(5):610-617.XIE X W,ZHAO Y R,SUN X Y,et al.Identification and control effect of a biocontrol bacterium against Phytophthora root rot of tomato[J].Acta Phytopathologica Sinica,2020,50(5):610-617.
- [11]邹修为,熊有明,谢剑波,等.一株辣椒疫霉拮抗菌Y4-39分离鉴定及生防作用研究[J].湖南农业科学,2022(6):1-5.ZOU X W,XIONG Y M,XIE J B,et al.Isolation and identification of an antagonistic strain Y4-39 and its application for biocontrolling Phytophthora capsici[J].Hunan Agricultural Sciences,2022(6):1-5.
- [12]潘培培,刘东平,谢太震,等.辣椒疫霉菌拮抗细菌L14-3的生防潜力及其鉴定[J].中国瓜菜,2021,34(1):29-34.PAN P P,LIU D P,XIE T Z,et al.Biocontrol potential of antagonistic bacteria L14-3 against Phytophthora capsici and its identification[J].China Cucurbits and Vegetables,2021,34(1):29-34.
- [13]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001.DONG X Z,CAI M Y.Handbook of systematic identification of common bacteria[M].Beijing:Science Press,2001.
- [14]张婉菊,谢太震,陈梦多,等.根际细菌LK2-3对黄瓜枯萎病的生物防治作用[J].中国瓜菜,2022,35(7):25-30.ZHANG W J,XIE T Z,CHEN M D,et al.Biocontrol effect of rhizosphere bacteria LK2-3 against Fusarium wilt of cucumber[J].China Cucurbits and Vegetables,2022,35(7):25-30.
- [15]张宁,张晶晶,李雅淑,等.人参黑斑病生防菌的分离鉴定及防效[J].中国生物防治学报,2022,38 (5):1308-1315.ZHANG N,ZHANG J J,LI Y S,et al.Isolation,identification and control effect of biocontrol bacteria against ginseng black spot disease[J].Chinese Journal of Biological Control,2022,38(5):1308-1315.
- [16]SCHWYN B,NEILANDS J B.Universal chemical assay for the detection and determination of siderophores[J].Analytical Biochemistry,1987,160(1):47-56.
- [17]徐婷,朱天辉,李姝江,等.贝莱斯芽孢杆菌Bacillus velezensis YB15 β-葡聚糖酶的抑菌作用与基因克隆[J].中国生物防治学报,2014,30(2):276-281.XU T,ZHU T H,LI S J,et al.Fungus-inhibitory activity and gene cloning of β-glucanase from Bacillus velezensis YB15[J].Chinese Journal of Biological Control,2014,30(2):276-281.
- [18]STAJKOVI? O,DELIC D,JOSIC D,et al.Improvement of common bean growth by co-inoculation with rhizobium and plant growth-promoting bacteria[J].Romanian Biotechnological Letters,2011,16(1):5919-5926.
- [19]KOZIK E,FOOLAD M R,JONES R A.Genetic analysis of resistance to Phytophthora root rot in tomato (Lycopersicon esculentum Mill.)[J].Plant Breeding,1991,106(1):27-32.
- [20]毛爱军,胡洽,耿三省.辣椒疫病菌接种鉴定技术研究[J].北京农业科学,1998,16(2):21-24.MAO A J,HU Q,GENG S S.Research on identification technology of Phytophthora capsici inoculation[J].Beijing Agricultural Sciences,1998,16(2):21-24.
- [21]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2005.GAO J F.Experimental guidance for plant physiology[M].Beijng:Higher Education Press,2005.
- [22]DAUD N S,ROSLI M A,AZAM Z M,et al.Paenibacillus polymyxa bioactive compounds for agricultural and biotechnological applications[J].Biocatalysis and Agricultural Biotechnology,2019,18:92-101.
- [23]张忠良,刘东平,潘培培,等.多粘类芽孢杆菌(Paenibacillus polymyxa) K18-5不同悬浮液处理对黄瓜枯萎病抑制作用的影响[J].河南农业大学学报,2019,53(5):724-730.ZHANG Z L,LIU D P,PAN P P,et al.Influence of inhibition effects of different suspensions treatments of Paenibacillus polymyxa (K18-5) against Fusarium wilt of cucumbe[J].Journal of Henan Agricultural University,2019,53(5):724-730.
- [24]陈雪丽,王光华,金剑,等.多粘类芽孢杆菌BRF-1和枯草芽孢杆菌BRF-2对黄瓜和番茄枯萎病的防治效果[J].中国生态农业学报,2008,16(2):446-450.CHEN X L,WANG G H,JIN J,et al.Biocontrol effect of Paenibacillus polymyxa BRF-1 and Bacillus subtilis BRF-2 on Fusarium wilt disease of cucumber and tomato[J].Chinese Journal of Eco-Agriculture,2008,16(2):446-450.
- [25]黄艺烁,谢学文,石延霞,等.多粘类芽胞杆菌ZF197对白菜茎基腐病防治效果[J].园艺学报,2020,47(6):1059-1071.HUANG Y S,XIE X W,SHI Y X,et al.Biocontrol effect of Paenibacillus polymyxa strain ZF197 against base stem rot of chinese cabbage[J].Acta Horticulturae Sinica,2020,47(6):1059-1071.
- [26]徐颖华,柴沙沙,李成阳等.多粘类芽胞杆菌分离鉴定及其对甘薯蔓割病的防治效果[J].南方农业学报,2022,53(4):1057-1065.XU Y H,CHAI S S,LI C Y,et al.Isolation and identification of Paenibacillus polymyxa strain and its control effect on Fusarium bulbigenum of sweet potato[J].Journal of Southern Agriculture,2022,53(4):1057-1065.
- [27]TAHERI E,TARIGHI S,Taheri P.Characterization of root endophytic Paenibacillus polymyxa isolates with biocontrol activity against Xanthomonas translucens and Fusarium graminearum[J].Biological Control,2022,174(11):503-515.
- [28]XU S J,KIM B S.Evaluation of Paenibacillus polymyxa strain SC09-21 for biocontrol of Phytophthora blight and growth stimulation in pepper plants[J].Tropical Plant Pathology,2016,41:162-168.
- [29]KLOEPPER J W,BEAUCHAMP C J.A review of issues related to measuring colonization of plant roots by bacteria[J].Canadian Journal of Microbiology,1992,38(12):1219-1232.
- [30]张亮,盛浩,袁红,等.荧光假单胞菌PEF-5#18防控番茄枯萎病的定殖机理[J].中国生物防治学报,2017,33(5):658-666.ZHANG L,SHENG H,YUAN H,et al.Colonization of Pseudomonas fluorescens PEF-5#18 to control Fusarium wilt disease on tomato[J].Chinese Journal of Biological Control,2017,33(5):658-666.
- [31]史洪丽,李腊,郭翠梅,等.番茄灰霉病生防菌株TL1的分离、鉴定及其生防能力分析[J].园艺学报,2023,50(1):79-90.SHI H L,LI L,GUO C M,et al.Isolation,Identification and analysis of biocontrol ability of biocontrol strain TL1against tomato Botrytis cinerea[J].Acta Horticulturae Sinica,2023,50(1):79-90.
- [32]冯丹,曹永清,刘艳,等.南方红豆杉根际促生细菌蜡状芽孢杆菌CLY07的接种效应[J].西南林业大学学报(自然科学),2022,42(1):30-36.FENG D,CAO Y Q,LIU Y,et al.Inoculation effect of growth-promoting rhizobacteria Bacillus cereus CLY07 of Taxus chinensis var.mairei[J].Journal of Southwest Forestry University (Natural Sciences),2022,42 (1):30-36.
- [33]常淑娴.寄主植物与普城沙雷菌A21-4定殖的相互影响[D].郑州:河南农业大学,2011.CHANG S X.The interaction of plants and Serratia plymuthica A21-4 colonization ability[D].Zhengzhou:Hennan Agricultural University,2011.
- [34]YUAN J,ZHAO J,WEN T,et al.Root exudates drive the soil-borne legacy of aboveground pathogen infection[J].Microbiome,2018,6(1):1-12.