曹亚兵,赵振利,翟晓巧,赵婉,邓敏捷,董焱鹏,范国强

• 1:河南农业大学泡桐研究所

摘要(Abstract)：

为阐明泡桐多倍体响应盐胁迫的分子机制,本试验以白花泡桐为材料,采用同位素相对标记与绝对定量技术(isobaric tags for relative and absolute quantitation,iTRAQ)结合液相色谱与串联质谱(liquid chromatography-tandem mass spectrometry,LC-MS/MS)定量蛋白质组技术,研究盐处理前后白花泡桐四倍体和对应二倍体的蛋白质表达变化。同时,选择6个差异表达蛋白质进行实时荧光定量PCR(qRT-PCR)验证。通过比对分析,共鉴定到2 851个蛋白质,有32个是倍性相关的盐应答差异蛋白质。KEGG pathway分析发现,这些差异蛋白质主要参与光合作用,信号转导,植物病原相互作用及淀粉和糖代谢等。其中,2-酮戊二酸脱氢酶E1、过氧化物酶和氢离子转运ATP合酶表达差异显著,表明这些蛋白质可能是潜在的盐应答蛋白质。QRT-PCR验证结果表明,mRNA与蛋白质表达水平不一致。

关键词(KeyWords)： 白花泡桐;多倍体;盐胁迫;蛋白质组;iTRAQ

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30571496);; 河南省科技厅科研单位基本业务费(2015JB01003)

作者(Author): 曹亚兵,赵振利,翟晓巧,赵婉,邓敏捷,董焱鹏,范国强

DOI: 10.16445/j.cnki.1000-2340.2016.05.006

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