李娜娜;杨彦芳;赵飞云;胡秀丽;

• 1:河南农业大学生命科学学院

摘要(Abstract)：

以玉米(Zea mays L.)品种郑单958幼苗第2个新展开的叶片为试验材料,运用双向电泳(2-DE)和质谱分析等技术,确定高温胁迫下与s HSP26相关蛋白及其与叶绿体关系。质谱鉴定结果显示,胶上存在24个蛋白质差异点,属于14个蛋白质,归为6种功能类别,其中42%蛋白质参与光反应。与其对照相比,用s HSP26抗体(AT-s HSP26)处理后,与光合作用相关蛋白(ATP合酶α,β和γ亚基、放氧增强子蛋白(OEE1)、叶绿素a/b结合蛋白、细胞色素b6/f复合体铁硫亚基、光系统Ⅰ反应中心亚基Ⅳ和铁硫中心蛋白)表达下调。并且叶绿体PSⅡ的放氧速率明显降低。表明s HSP26通过调控光合作用相关蛋白,从而在一定程度上对叶绿体起到保护作用,最终影响玉米叶绿体的耐热性。

关键词(KeyWords)： 玉米;s HSP26;叶绿体蛋白;双向电泳;高温胁迫

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31171470)

作者(Authors): 李娜娜;杨彦芳;赵飞云;胡秀丽;

DOI: 10.16445/j.cnki.1000-2340.2015.06.002

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