翁少亭,曹建科,常艳凯,张素梅

• 1:河南农业大学牧医工程学院
• 2:中国农业科学院兰州兽医研究所

摘要(Abstract)：

为了优化H1和H3亚型猪流感病毒(Swine Influenza Viruse,SIV)血清学检测方法,更准确了解猪流感病毒在中国猪群中的流行与分布情况,本试验制备了欧洲禽源猪H1N1病毒和人源猪H3N2病毒的血凝素特异性单因子血清。将人工合成的2种HA基因与p CAGGS真核表达载体做连接,构建HA基因的真核表达重组质粒。经酶切鉴定及体外瞬时表达检测证实重组质粒结构正确,能正常表达目的蛋白。大量提取纯化重组质粒,以每只鸡200μg的剂量肌肉接种SPF鸡,三免后收获鸡血清。通过血凝抑制试验检测HA单因子血清效价。试验结果显示,HA单因子血清抗体水平达到4log_2~6log_2。交叉HI试验结果显示,2个亚型血清间无交叉反应现象,特异性强。

关键词(KeyWords)： 猪流感病毒;H1亚型;H3亚型;单因子血清

Abstract:

Keywords:

基金项目(Foundation): 河南省科技开放合作项目(132106000065)

作者(Author): 翁少亭,曹建科,常艳凯,张素梅

DOI: 10.16445/j.cnki.1000-2340.2016.05.012

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