赵玉龙,李娜娜,赵飞云,胡秀丽

• 1:河南农业大学生命科学学院

摘要(Abstract)：

以玉米(Zea mays L.)品种郑单958为试验材料,利用免疫印迹等技术,确定干旱高温复合胁迫条件下,Ca~(2+)/CaM对sHSP26的表达、叶绿体抗氧化防护酶活性的影响。结果显示:(1)高温、高温干旱复合胁迫下sHSP26的表达量明显高于对照和干旱,经CaCl_2预处理后能显著增强sHSP26的表达;(2)经CaCl_2预处理后,叶绿体内超氧化物歧化酶(SOD)、谷胱甘肽还原酶(GR)和抗坏血酸过氧化物酶(APX)活性显著增加,而经Ca~(2+)螯合剂EGTA及CaM抑制剂TFP和W7处理后,这些抗氧化防护酶活性显著下降。本研究表明,在干旱高温复合胁迫条件下适宜浓度的Ca~(2+)能够增强sHSP26的表达及提高叶绿体抗氧化防护酶活性,从而对叶绿体起到保护作用,提高植物耐热性。

关键词(KeyWords)： 干旱高温胁迫;免疫印记;sHSP26;叶绿体抗氧化防护酶

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31171470);; 河南省高校科技创新人才支持计划(13HASTIT001ABA)

作者(Author): 赵玉龙,李娜娜,赵飞云,胡秀丽

DOI: 10.16445/j.cnki.1000-2340.2016.04.001

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