林章萍,陈能海,崔波,吕海林,翁泽斌,刘泓

• 1:福建农林大学资源与环境学院
• 2:福建农林大学生命科学学院

摘要(Abstract)：

选用模式植物拟南芥为材料,研究其在三环多环芳烃菲胁迫下的差异表达蛋白编码基因的转录水平表达。研究显示,甲基化酶类蛋白编码基因(At5g17920,At1g11860,At1g50480)、活性氧相关基因(At1g65980,At2g47730,At3g54660)、信号传导类蛋白编码基因(At4g11010,AT1g18080)、植物防卫相关蛋白编码基因(At3g23490,At1g54010,At3g14210,At3g16420)、糖分解代谢的蛋白编码基因(At3g52930,At2g36460,At1g04410)和氨基酸代谢相关酶基因(At5g19550,At2g30970)在开始受到菲胁迫时表达上调,大部分在24 h出现表达高峰,甚至48 h时出现2次表达高峰;而活性氧相关基因(At3g52960)和糖分解代谢的编码基因(At3g01500,At1g48030)的表达则从12 h到72 h都受到抑制。结果表明,不同时间菲胁迫下,各基因转录水平出现显著差异,且同一功能类型中的各个基因在响应PAHs(菲)胁迫过程中参与程度不同。上调的编码基因表现出胁迫24h的表达峰值,反映出菲胁迫24 h植物RNA水平的响应达到高峰,随着胁迫时间的延长,表达量不同程度下降。

关键词(KeyWords)： 拟南芥;多环芳烃;菲;早期响应;应答机制

Abstract:

Keywords:

基金项目(Foundation): 福建省自然科学基金资助项目(2014J01089);; 福州市科技计划资助项目(2013-G-106)

作者(Author): 林章萍,陈能海,崔波,吕海林,翁泽斌,刘泓

DOI: 10.16445/j.cnki.1000-2340.2016.01.014

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