汪敏,赵静,李洪连,谷素静,马宗斌,焦睿

• 1:河南农业大学植物保护学院
• 2:河南农业大学农学院
• 3:河南农业大学生命科学学院

摘要(Abstract)：

为了获得大丽轮枝菌(Verticillium dahliae)微菌核缺陷突变体,利用农杆菌介导转化大丽轮枝菌,共获得270个T-DNA随机插入突变体,从中筛选出了4个气生菌丝发达、不产微菌核的突变体,利用高效TAIL-PCR技术,获得了其侧端序列,结合生物信息学方法,获得4个突变体T-DNA在大丽轮枝菌基因组插入位点的基因信息。

关键词(KeyWords)： 大丽轮枝菌;棉花黄萎病;T-DNA插入;微菌核

Abstract:

Keywords:

基金项目(Foundation): 河南省棉花产业技术体系建设专项资金资助项目(S2013-07-G04);; 河南省教育厅科学技术研究重点项目(14A210025)

作者(Author): 汪敏,赵静,李洪连,谷素静,马宗斌,焦睿

DOI: 10.16445/j.cnki.1000-2340.2015.01.014

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