RAPD技术在产气荚膜杆菌基因分型中的应用研究Genomic parting analyses of Clostridium perfringens by randomly amplified polymorphic DNA
杨明凡,田永军,崔保安,张自富,王学斌
摘要(Abstract)：
采用随机扩增多态性DNA(RondomlyAmplifiedPolymorphicDNA ,RAPD)技术对产气荚膜杆菌进行PCR扩增。结果产气荚膜杆菌A ,B ,C ,D 4个型均可扩增出约 430bp的条带 ,B ,C型还扩增出约 2 10bp条带 ,B ,D型还扩增出约 12 10bp条带 ,A型还扩增出约 2 40bp和 6 90bp条带 ,各型之间的条带差别明显 ,从而为产气荚膜杆菌的基因分型、PCR快速诊断提供可靠的依据
关键词(KeyWords)： 产气荚膜杆菌;随机扩增多态性DNA;基因;分型
基金项目(Foundation): “九五”国家科技攻关项目(960030308)
作者(Author): 杨明凡,田永军,崔保安,张自富,王学斌
DOI: 10.16445/j.cnki.1000-2340.2000.01.024
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