天然脂溶性抗氧化剂对亚麻籽油氧化稳定性的影响Effect of natural fat-soluble antioxidants on oxidative stability of flaxseed oil
马燕,孙宏涛,乔明武,宋莲军,郭洪涛,孙晓冬,黄现青
摘要(Abstract):
【目的】探究天然脂溶性抗氧化剂单独使用、复配使用和增效使用时对亚麻籽油氧化稳定性的影响,以提高亚麻籽油的氧化稳定性,延长其货架期。【方法】选取茶多酚棕榈酸酯、迷迭香提取物和抗坏血酸棕榈酸酯3种天然脂溶性抗氧化剂,植酸和柠檬酸为增效剂,以保护系数(protection factor,Pf)为衡量指标,在单一抗氧化剂使用基础上,采用响应面法优化3种抗氧化剂最佳复配配方,在此基础上使用增效剂对其抗氧化效果进行增强并预测货架期。【结果】抗氧化剂单独使用对亚麻籽油氧化稳定性均有显著性影响,但与合成抗氧化剂(阳性对照组)相比效果较差;最佳复配配方为茶多酚棕榈酸酯0.21 g·kg~(-1)、迷迭香提取物0.45 g·kg~(-1),此时Pf为1.87,氧化稳定性较好;增效剂确定为柠檬酸0.20 g·kg~(-1);将最终复配配方应用于亚麻籽油中并预测25℃下亚麻籽油货架期为843 d,是添加合成抗氧化剂(阳性对照组)的2倍,同时是未添加抗氧化剂(空白对照组)的10倍。【结论】复配配方显著提高了亚麻籽油氧化稳定性,延长了亚麻籽油货架期,为取代合成抗氧化剂提供了理论基础,促进亚麻籽油工业化开发和应用领域拓展。
关键词(KeyWords): 亚麻籽油;氧化稳定性;抗氧化剂;增效剂;货架期
基金项目(Foundation): 中国博士后科学基金资助项目(2022M711460);; 河南省研究生联合培养基地项目(YJS2022JD16);; 河南省高校科技创新团队支持计划(23IRTSTHN023)
作者(Author): 马燕,孙宏涛,乔明武,宋莲军,郭洪涛,孙晓冬,黄现青
DOI: 10.16445/j.cnki.1000-2340.20220921.001
参考文献(References):
- [1] 祁惠芳,程子良,孔维宝,等.亚麻籽有效成分的提取及其综合利用研究进展[J].中国油脂,2019,44(11):102-107.QI H F,CHENG Z L,KONG W B,et al.Progress in extraction and comprehensive utilization of effective components in flaxseed[J].China Oils and Fats,2019,44(11):102-107.
- [2] DERBYSHIRE E.Brain health across the lifespan:a systematic review on the role of Omega-3 fatty acid su-pplements[J].Nutrients,2018,10(8):1094-1112.
- [3] LAYé S,NADJAR A,JOFFRE C,et al.Anti-inflammatory effects of Omega-3 fatty acids in the brain:Phy-siological mechanisms and relevance to pharmacology[J].Pharmacological Reviews,2018,70(1):12-38.
- [4] GOYAL A,SHARMA V,UPADHYAY N,et al.Flax and flaxseed oil:An ancient medicine & modern functional food[J].Journal of Food Science and Technology,2014,51(9):1633-1653.
- [5] D'ELISEO D,VELOTTI F.Omega-3 fatty acids and cancer cell cytotoxicity:Implications for multi-targeted cancer therapy[J].Journal of Clinical Medicine,2016,5(2):15-29.
- [6] 于坤,禹晓,程晨,等.制油工艺对亚麻籽油品质及脂质伴随物含量的影响[J].食品科学,2020,41(16):233-243.YU K,YU X,CHENG C,et al.Effects of processing techniques on the quality properties and lipid concomitants of flaxseed oil[J].Food Science,2020,41(16):233-243.
- [7] UPADHYAY R,MISHRA H N.Effect of relative humidity and light conditions on the oxidative stability of sunflower oil blends stabilised with synthetic and natural antioxidants[J].International Journal of Food Science and Technology,2016,51(2):293-299.
- [8] DOLATABADI J E N,KASHANIAN S.A review on DNA interaction with synthetic phenolic food additives[J].Food Research International,2010,43(5):1223-1230.
- [9] TAGHVAEI M,JAFARI S M.Application and stability of natural antioxidants in edible oils in order to substitute synthetic additives[J].Journal of Food Science and Technology,2015,52(3):1272-1282.
- [10] 武州,赵慧敏,惠菊,等.抗坏血酸棕榈酸酯对玉米油储藏稳定性的影响[J].中国油脂,2021,46(9):108-111.WU Z,ZHAO H M,HUI J,et al.Effect of ascorbyl palmitate on the storage stability of maize oil[J].China Oils and Fats,2021,46(9):108-111.
- [11] WANG Y,ZHOU S,JIANG Y R,et al.Effects of tea polyphenol palmitate on the crystallization of rice bran oil[J].Journal of Food Engineering,2021,292:110279.
- [12] OH W Y,SHAHIDI F.Lipophilization of resveratrol and effects on antioxidant activities[J].Journal of Agricultural and Food Chemistry,2017,65(39):8617-8625.
- [13] CHOE E,MIN D B.Mechanisms of antioxidants in the oxidation of foods[J].Comprehensive Reviews in Food Science and Food Safety,2009,8(4):345-358.
- [14] OMAR K A,SHAN L,WANG Y L,et al.Stabilizing flaxseed oil with individual antioxidants and their mixtures[J].European Journal of Lipid Science and Technology,2010,112(9):1003-1011.
- [15] 龚欣.抗坏血酸棕榈酸酯的合成及应用研究[D].广州:华南理工大学,2014.GONG X.Preparation and application of L-ascorbyl palmitate[D].Guangzhou:South China University of Technology,2014.
- [16] PARK H,RHO H,KIM D H,et al.Modified rancimat method for evaluation of antioxidative effect against skin lipids[J].Bulletin of the Korean Chemical Society,2010,31(6):1751-1752.
- [17] 王雅娟,刘君,马传国.剪切与均质条件和抗氧化剂对水包剥离亚麻籽油乳液稳定性的影响[J].河南工业大学学报(自然科学版),2022,43(2):40-47.WANG Y J,LIU J,MA C G.The effects of shear and homogenization conditions and antioxidants on the physicochemical stability of stripped flaxseed oil in water food emulsion[J].Journal of Henan University of Technology (Natural Science Edition),2022,43(2):40-47.
- [18] 亢敏,党玲.精炼工艺对亚麻籽油品质及其氧化稳定性的影响[J].粮食与油脂,2021,34(6):74-77.KANG M,DANG L.Effects of refining process on qua-lity and oxidation stability of linseed oil[J].Cereals & Oils,2021,34(6):74-77.
- [19] 于坤.内源性脂质伴随物对亚麻籽油及其纳米乳液稳定性的影响研究[D].北京:中国农业科学院,2020.YU K.Effect of lipid concomitants on the stability of flaxseed oil and oil-in-water nanoemulsions[D].Beijing:Chinese Academy of Agricultural Sciences,2020.
- [20] 国家质量监督检验检疫总局,中国国家标准化管理委员会.动植物油脂氧化稳定性的测定:GB/T 21121—2007[S].北京:中国标准出版社,2008.General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China,Standardization Administration of the People's Republic of China.Animal and vegetable fats and oils Determination of oxidation stability(Accelerated oxidation test):GB/T 21121—2007[S].Beijing:Standards Press of China,2008.
- [21] LU T,SHEN Y,WANG J H,et al.Stabilizing flaxseed oil with individual antioxidants and their mixturesantioxidants[J].Journal of Food Processing and Preservation,2020,44(3):e14355.
- [22] 孙少忆,马露,刘军,等.葡萄酒渣多酚类物质超声波辅助提取工艺优化及其抗氧化活性研究[J].河南农业大学学报,2021,55(2):328-337.SUN S Y,MA L,LIU J,et al.Optimization of ultrasonic assisted extraction of polyphenols from grape wine residue and its antioxidant activity[J].Journal of Henan Agricultural University,2021,55(2):328-337.
- [23] 周旭.几种油溶性天然抗氧化剂在核桃油、葡萄籽油中的应用研究[J].中国油脂,2017,42(3):64-68.ZHOU X.Application of oil soluble natural antioxidants in walnut oil and grape seed oil[J].China Oils and Fats,2017,42(3):64-68.
- [24] 中华人民共和国国家卫生和计划生育委员会.食品安全国家标准食品添加剂使用标准:GB 2760—2014[S].北京:中国标准出版社,2015.National Health and Family Planning Commission of the P.R.C.National food safety standard the standard for the use of food additives:GB 2760—2014[S].Beijing:Standards Press of China,2015.
- [25] RUDNIK E,SZCZUCINSKA A,GWARDIAK H,et al.Comparative studies of oxidative stability of linseed oil[J].Thermochimica Acta,2001,370(1/2):135-140.
- [26] 陈国艳,陈若馨,聂绪恒,等.抗氧化剂对青刺果油氧化稳定性的影响[J].粮食与食品工业,2021,28(2):31-34.CHEN G Y,CHEN R X,NIE X H,et al.The effect of antioxidant on the oxidation stability of Prinsepia utilis rogle oil[J].Cereal & Food Industry,2021,28(2):31-34.
- [27] 易志.亚麻籽油贮藏稳定性研究[D].广州:华南农业大学,2016.YI Z.Study on storage stability of linseed oil[D].Guangzhou:South China Agricultural University,2016.
- [28] 詹永,廖霞,杨勇,等.宛艾多酚类物质的提取工艺优化及组分分析[J].河南农业大学学报,2021,55(1):97-105.ZHAN Y,LIAO X,YANG Y,et al.Optimization of extraction process and component analysis of polyphenols from Wan'ai[J].Journal of Henan Agricultural University,2021,55(1):97-105.
- [29] 高浩源,李苗云,赵莉君,等.不同乳化剂对牛骨白汤稳定性的影响[J].河南农业大学学报,2022,56(2):290-300.GAO H Y,LI M Y,ZHAO L J,et al.Effect of different emulsifiers on the stability of beef bone white soup[J].Journal of Henan Agricultural University,2022,56(2):290-300.
- [30] BREWER M S.Natural antioxidants:Sources,compounds,mechanisms of action,and potential applications[J].Comprehensive Reviews in Food Science and Food Safety,2011,10(4):221-247.
- [31] 周旭.脂溶性天然抗氧化剂在葡萄籽油和核桃油中的应用研究[D].杭州:浙江大学,2016.ZHOU X.Study on the application of oil-soluble natural antioxidants in grape seed oil and walnut oil[D].Hangzhou:Zhejiang University,2016.
- [32] 高荫榆,雷占兰,谢何融,等.L-抗坏血酸棕榈酸酯的抗氧化效果研究[J].食品科学,2007,28(11):60-62.GAO Y Y,LEI Z L,XIE H R,et al.Study on antioxidation effect of ascorbyl palmitate[J].Food Science,2007,28(11):60-62.
- [33] LAGUERRE M,BAYRASY C,PANYA A,et al.What makes good antioxidants in lipid-based systems?The next theories beyond the polar paradox[J].Critical Reviews in Food Science and Nutrition,2015,55(2):183-201.
- [34] 郭彤,于凤芝,胡平,等.维生素E调节抗氧化作用的研究进展[J].山东畜牧兽医,2021,42(12):42-49.GUO T,YU F Z,HU P,et al.Research progress of vitamin E regulating antioxidant effect[J].Shandong Journal of Animal Science and Veterinary Medicine,2021,42(12):42-49.
- [35] MELO P S,ARRIVETTI L,ALENCAR S M,et al.Antioxidative and prooxidative effects in food lipids and synergism with α-tocopherol of a?aí seed extracts and grape Rachis extracts[J].Food Chemistry,2016,213:440-449.
- [36] 雷琳.L-抗坏血酸棕榈酸酯的抗氧化性研究[J].中国医药导报,2009,6(17):13-14.LEI L.Study on the antioxidation of L-ascorbyl palmitate[J].China Medical Herald,2009,6(17):13-14.
- [37] MOHANAN A,NICKERSON M T,GHOSH S.Oxidative stability of flaxseed oil:Effect of hydrophilic,hydrophobic and intermediate polarity antioxidants[J].Food Chemistry,2018,266:524-533.