张淑红,包格日乐,李治国,陈红歌

• 1:河南农业大学生命科学学院
• 2:商丘师范学院生命科学学院
• 3:商丘师范学院环境与规划学院

摘要(Abstract)：

通过纯培养和高通量测序,对喀拉昆仑南部熊彩岗日地区冰川雪样和水样细菌多样性进行了研究。纯培养结果表明,冰川细菌由Bacteroidetes、Actinobacteria、Deinococcus-Thermus、Proteobacteria4个门组成,属水平由Pseudomonas、Janthinobacterium、Flavobacterium、Devosia、Duganella、Deinococcus、Cryobacterium、Rugamonas、Arthrobacter、Mycetocola、Massilia、Salinibacterium组成;高通量测序结果表明,冰川细菌主要由Proteobacteria、Bacteroidetes、Firmicutes 3个门组成,由Acidovorax、Bacillus、Bacteroides、Brevundimonas、Herbaspirillum、Massilia、Pedobacter、Phenylobacterium、Polaromonas、Pseudorhodobacter、Variovorax等属组成。2种方法综合起来更能全面地分析细菌群落信息。雪环境和水环境的细菌多样性差异不大。细菌群落组成既有重复的,也有各自环境所特有的。同时也检测到了熊彩岗日地区冰川所特有的细菌类群。

关键词(KeyWords)： 细菌;多样性;冰川;熊彩岗日地区

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31100369,41101072,41330526)

作者(Author): 张淑红,包格日乐,李治国,陈红歌

DOI: 10.16445/j.cnki.1000-2340.2016.02.022

参考文献(References)：

• [1]陈勇.青藏高原冰雪微生物多样性及其与气候环境关系的研究[D].兰州:兰州大学,2009.
• [2]张淑红,侯书贵,秦翔,等.祁连山老虎沟12号冰川退缩对细菌优势种群影响的初步研究[J].冰川冻土,2013,35(3):751-760.
• [3]ZHANG S,HOU S,QIN X,et al.Preliminary study on effects of glacial retreat on the dominant glacial snow bacteria in Laohugou Glacier No.12[J].Geomicrobiology Journal,2015,32(2):113-118.
• [4]LI Z,FANG H,TIAN L,et al.Changes in the glacier extent and surface elevation in Xiongcaigangri region,Southern Karakoram Mountains,China[J].Quaternary International,2015,371(12):67-75.
• [5]ZHOU J,BRUNS M A,TIEDJE J M.DNA recovery from soils of diverse composition[J].Applied and Environmental Microbiology,1996,62(2):461-468.
• [6]CHAKRAVORTY S,HELB D,BURDAY M,et al.A detailed analysis of 16S ribosomal RNA gene segments for the diagnosis of pathogenic bacteria[J].Journal of Microbiological Methods,2007,69(2):330-339.
• [7]CAPORASO J G,KUCZYNSKI J,STOMBAUGH J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nature Methods,2010,7(5):335-336.
• [8]EDGAR R C,HASS B J,CLEMENTE J C,et al.UCHIME improves sensitivity and speed of chimera detection[J].Bioinformatics,2011,27(16):2194-2200.
• [9]WANG Q,GARRITY G M,TIEDJEi J M,et al.Naive Bayesian classifier for rapid assignment of r RNA sequences into the new bacterial taxonomy[J].Applied and Environmental Microbiology,2007,73(16):5261-5267.
• [10]QUAST C,PRUESSE E,YILMAZ P,et al.The SILVA ribosomal RNA gene database project:improved data processing and web-based tools[J].Nucleic Acids Research,2013,41(D1):D590-D596.
• [11]AMATO K R,YEOMAN C J,KENT A,et al.Habitat degradation impacts black howler monkey(Alouatta pigra)gastrointestinal microbiomes[J].The ISME Journal,2013,7(7):1344-1353.
• [12]SCHLOSS P D,GEVERS D,WEATCOTT S L.Reducing the effects of PCR amplification and sequencing artifacts on 16S r RNA-based studies[J].PLo S One,2011,6(12):e27310.
• [13]OBERAUNER L,ZACHOW C,LACKNER S,et al.The ignored diversity:complex bacterial communities in intensive care units revealed by 16S pyrosequencing[J].Scientific Reports,2013,3(5-6):413-1424.
• [14]WU X,ZHANG W,LIU G,et al.Bacterial diversity in the foreland of the Tianshan Glacier No.1,China[J].Environmental Research Letters,2012,7(1):1-9.
• [15]SHEN L,LIU Y,WANG N,et al.Massilia yuzhufengensis,isolated from an ice core[J].International Journal of Systematic and Evolutionary Microbiology,2012,64(4):1285-1290.
• [16]HUANG J P,SWAIN A K,THACKER R W,et al.Bacterial diversity of the rock-water interface in an East Antarctic freshwater ecosystem,Lake Tawani(P)+[J].Aquatic Biosystems,2013,9:4.doi:10.1186/2046-9063-9-4.
• [17]SCHTTE U M,ABDO Z,FOSTER J,et al.Bacterial diversity in a glacier foreland of the high Arctic[J].Molecular Ecology,2010,19(Suppl.1):54-66.
• [18]LIU Y,YAO T,JIAO N,et al.Bacterial diversity in the snow over Tibetan Plateau Glaciers[J].Extremophiles,2009,13(3):411-423.
• [19]ZHANG S,HOU S,MA X,et al.Culturable bacteria in Himalayan glacial ice in response to atmospheric circulation[J].Biogeosciences,2007,4:1-9.
• [20]XIANG S,YAO T,AN L,et al.Change of bacterial community in the Malan ice core and its relation to climate and environment[J].Chinese Science Bulletin,2004,49(17):1869-1875.
• [21]XIANG S,YAO T,AN L,et al.Bacterial diversity in Malan ice core from the Tibetan Plateau[J].Folia Microbiologica,2004,49(3):269-275.
• [22]SRINIVAS T N,SINGH S M,PRADHAN S,et al.Comparison of bacterial diversity in proglacial soil from Kafni Glacier,Himalayan Mountain ranges,India,with the bacterial diversity of other glaciers in the world[J].Extremophiles,2011,15(6):673-690.
• [23]ZHANG S,YANG G,WANG Y,et al.Abundance and community of snow bacteria from three glaciers in the Tibetan Plateau[J].Journal of Environmental Sciences,2010,22(9):1418-1424.
• [24]MITEVA V I,SHERIDAN P P,BRENCHLEY J E.Phylogenetic and physiological diversity of microorganisms isolated from a deep Greenland glacier ice core[J].Applied and Environmental Microbiology,2004,70(1):202-213.
• [25]GIUDICE A L,CARUSO C,MANGANO S,et al.Marine bacterioplankton diversity and community composition in an Antarctic coastal environment[J].Microbial Ecology,2012,63(1):210-223.
• [26]LAROSE C,BERGER S,FERRARI C,et al.Microbial sequences retrieved from environmental samples from seasonal Arctic snow and meltwater from Svalbard,Norway[J].Extremophiles,2010,14(2):205-212.
• [27]LIU Y,YAO T,JIAO N,et al.Microbial diversity in the snow,a moraine lake and a stream in Himalayan glacier[J].Extremophiles,2011,15(3):411-421.