李永丽,周洲,尹新明

• 1:信阳农林学院农学院
• 2:河南农业大学植物保护学院

摘要(Abstract)：

【目的】在长光照和短光照条件下，桃小食心虫Carposina sasakii Matsumura脱果老熟幼虫分别进行非滞育和滞育发育，探究保幼激素(JH)在2种发育方向调控中的差异，以及该差异过程JH合成代谢相关基因表达的分子基础。【方法】试验首先采用Illumina HiSeqTM 4000测序平台测定了2种生长条件下老熟幼虫头部转录组，qPCR验证转录组数据的准确性，并筛选差异表达的JH合成代谢相关基因；然后结合2种发育方向JH丰度变化差异，进一步分析这些JH相关基因在此过程中的作用。【结果】试验共获得了39 330 876条序列读取片段，有效转录组数据44.35 Gb (SRA登录号：PRJNA495711),组装拼接获得56 723个单基因簇(unigene),平均长度963 bp,其中1 kb以上的unigene有16 343条，经过BLASTX比对成功注释27 180个unigene。2种光照条件下桃小脱果老熟幼虫JH丰度无显著差异；长光照桃小老熟幼虫进行非滞育发育，2 d后进入长茧，虫体JH丰度比初脱果少量增加；而短光照老熟幼虫滞育发育进入圆茧，JH含量比2 d前初脱果增加了3倍，JH丰度显著高于同时期长茧内幼虫。2种光照下脱果老熟幼虫存在993个差异表达基因，包括7个JH合成代谢相关基因；其中jhamt在短光照老熟幼虫表达量是长光照的3.4倍，jheh1、jheh2、jheh3和jheh4在短光照老熟幼虫头部表达量分别是长光照的19.6、1.9、4.3和0.4倍，jhbp1和jhbp2在的2.7和20.5倍。【结论】研究获得了桃小2种光周期下老熟幼虫高质量的转录组数据，明确了桃小进入圆茧滞育需要JH丰度大幅升高的过程，筛选并分析了JH合成代谢相关的7个差异表达基因，为进一步研究桃小进入滞育过程中JH调控的分子机制奠定了基础。

关键词(KeyWords)： 桃小食心虫;转录组;基因注释;保幼激素;光周期;滞育

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(31870638);; 国家现代农业产业技术体系资助项目(CARS-27);; 信阳农林学院高水平科研孵化器建设基金项目(FCL202109);信阳农林学院“作物绿色防控与品质调控”科技创新团队建设项目

作者(Author): 李永丽,周洲,尹新明

DOI: 10.16445/j.cnki.1000-2340.20211022.001

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