付濛濛,石中亚,李艺琳,叶霞,郑先波,谭彬,冯建灿

• 1:河南农业大学园艺学院

摘要(Abstract)：

为探究乙烯在葡萄果实生长发育期和采后的释放规律,及其乙烯受体Vv ETRs基因的表达模式,本研究利用气相色谱仪分别测定了‘阳光玫瑰’整个生长发育期和采后的果粒和穗轴的乙烯释放量,利用荧光定量RTqPCR的方法检测了每个时期葡萄果粒和穗轴中乙烯受体Vv ETR基因家族的表达量。结果表明,葡萄果粒和穗轴乙烯释放的变化模式完全不同,葡萄果粒一直处于低乙烯释放水平,均低于0.12μL·kg~(-1)·h~(-1),随着果实的成熟,乙烯释放量趋近于0;葡萄穗轴的乙烯释放量明显高于果粒,在花后14和70 d存在乙烯释放峰值,分别为2.67和1.71μL·kg~(-1)·h~(-1)。果粒和穗轴中的Vv ETRs的表达模式不同,果粒中乙烯受体在生长发育前期表达量较高,而穗轴中乙烯受体则是在生长发育后期及采后表达量较高,且变化范围较大。果粒中受体基因的表达量变化受到穗轴乙烯的诱导和影响。果粒中乙烯受体基因Vv ETR4与乙烯释放量之间呈显著负相关关系,而穗轴中乙烯受体基因Vv ETR2与乙烯释放量之间呈显著正相关关系。

关键词(KeyWords)： 葡萄;乙烯;乙烯受体基因;果粒;穗轴

Abstract:

Keywords:

基金项目(Foundation): 河南省自然科学基金项目(162300410151);河南省自然科学基金项目(30601613);; 河南省重大科技专项项目(151100110900);; 河南省现代农业产业技术体系建设专项项目(S2014-11-G02)

作者(Author): 付濛濛,石中亚,李艺琳,叶霞,郑先波,谭彬,冯建灿

DOI: 10.16445/j.cnki.1000-2340.2018.06.007

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