水稻强弱势粒24-nt siRNA表达量与DNA甲基化的关系研究Relationship between 24-nt siRNA expression and DNA methylation status in rice superior and inferior grains
刘燕霞;彭廷;赵亚帆;杜彦修;张静;李俊周;赵全志;孙红正;
摘要(Abstract):
24-nt siRNA参与指导DNA甲基化,为研究水稻强弱势子粒24-nt siRNA与DNA甲基化水平之间的关系,采用亚硫酸盐测序法检测强弱势子粒中DNA甲基化状态.结果表明,弱势粒24-nt siRNA表达量高于强势粒,而DNA甲基化水平低于强势粒.在CG,CHG和CHH等DNA甲基化中,CG类甲基化水平在强弱势粒间没有差异,而CHG和CHH类均存在较大差异,均表现为强势粒甲基化水平高于弱势粒.强弱势粒间CHG和CHH类甲基化差异是导致DNA甲基化差异的主要原因,而弱势粒24-nt siRNA表达量高可能是由于甲基化水平低造成的.
关键词(KeyWords): 水稻;强势粒;弱势粒;24-nt siRNA;DNA甲基化
基金项目(Foundation): 国家自然科学基金资助项目(31200973)
作者(Authors): 刘燕霞;彭廷;赵亚帆;杜彦修;张静;李俊周;赵全志;孙红正;
DOI: 10.16445/j.cnki.1000-2340.2014.01.009
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