49份小麦种质资源中Glu-1,Glu-3,Gli-1位点基因组成分析Compositions of Glu-1,Glu-3 and Gli-1 Alleleic Variation of Storage Protein in 49 Wheat Landracs
王瑞,张改生,王宏,F J ZELLER,S L K HSAM
摘要(Abstract)：
采用分步法SDS-PAGE和改良A-PAGE分析了49份小麦农家品种等资源的Glu-1,Glu-3,Gli-1位点基因组成.结果表明,Glu-1位点编码的HMW-GS组成共检测到14种图谱,其中占比例最大图谱(N,7+8,2+12)达44.9%,其次是(N,7+9,2+12)和(1,7+8,2+12)各占14.3%,(1,7+9,5+10)和(2*,7+9,2+12)各占4.1%,其余9种图谱各占2.0%;Glu-3位点编码的LMW-GS组成共检测到25种图谱,(a,j,c)占12.2%,(a,g,c)占10.2%,(new,g,c)占8.2%,(a,g,a),(a,j,a),(f,g,c)和(d,j,a)各占6.1%,(f,j,a),(d,g,c),(a,f,c)和(f,f,c)各占4.1%,其余14种图谱各占2.0%,Glu-A3位点新发现的基因图谱占14.3%;Gli-1位点编码的醇溶蛋白组成共检测到40种图谱,(a,f,l)和(f,l,i)各占6.1%,(f,l,a),(new,c,i),(b,g,l)和(l,l,a)各占4.1%,其它34种图谱各占2.0%,Gli-1位点新发现的基因图谱占16.3%;扬白麦、迟亚草、葫芦头麦、红秃头、红粒白芒、红火麦、矮孟牛IV中在Glu-3或Gli-1位点含有新发现的等位基因.
关键词(KeyWords)： 小麦;谷蛋白;醇溶蛋白
基金项目(Foundation): 陕西省科技攻关项目(2003-k01-05);; 西北农林科技大学育种专项(05Y2004-2)
作者(Author): 王瑞,张改生,王宏,F J ZELLER,S L K HSAM
DOI: 10.16445/j.cnki.1000-2340.2008.04.014
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