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【目的】鉴定影响红花种子中脂肪酸发育的关键基因，为进一步解析红花脂肪酸合成的分子机制提供依据。【方法】以花后4、10、16、22 d的红花种子(分别表示为D4、D10、D16、D22)为材料，测定不同花后时期种子37种脂肪酸含量，利用转录组测序和生物信息学分析方法鉴定差异表达基因，通过基因功能分类体系(gene ontology,GO)和京都基因与基因组百科全书(kyoto encyclopedia ofgenes and genomes,KEGG)富集分析，挖掘红花种子不同生长阶段的差异表达基因参与的生物学过程和生化代谢途径。【结果】红花种子发育前期饱和脂肪酸占总脂肪酸的56.52%，花后第10天以后，亚油酸质量分数由5.03%增长至75.41%。KEGG分析显示，D4～D10是红花脂肪酸合成的关键时期，有37个差异表达基因富集在不饱和脂肪酸的生物合成通路上，编码产物涉及硬脂酰辅酶A去饱和酶、omega-6脂肪酸去饱和酶、极长链烯酰辅酶A还原酶、乙酰辅酶A酰基转移酶。【结论】鉴定到29个基因参与红花种子脂肪酸代谢过程，发现了ACC、FATA、DGAT1和FAD2酶基因的表达对红花种子脂肪酸的累积具有促进作用。
Abstract：【Objective】To identify the key genes affecting fatty acid development in safflower seeds, so as to provide a theoretical basis and technical support for a deeper understanding of the molecular mechanisms of safflower fatty acid synthesis.【Method】Safflower seeds collected at 4, 10, 16, and 22 days after flowering(referred to as D4, D10, D16, and D22, respectively) were used as materials.The contents of 37 fatty acids in seeds at different developmental stages were measured.Transcriptome sequencing and bioinformatics analysis methods were employed to identify differentially expressed genes.Gene ontology(GO) and kyoto encyclopedia of genes and genomes(KEGG) enrichment analyses were conducted to explore the biological processes and biochemical metabolic pathways in which the differentially expressed genes in safflower seeds at different growth stages were involved.【Result】n the early stage of safflower seed development, saturated fatty acids accounted for 56.52% of the total fatty acids.After the 10 th day after flowering, the content of linoleic acid increased from 5.03% to 75.41%.KEGG analysis indicated that the period from D4 to D10 was the critical stage for safflower fatty acid synthesis, with 37 differentially expressed genes enriched in the biosynthetic pathway of unsaturated fatty acids.The encoded products included stearoyl-CoA desaturase, omega-6 fatty acid desaturase, very-long-chain enoyl-CoA reductase, and acetyl-CoA acyltransferase.【Conclusion】A total of 29 genes involved in the fatty acid metabolic process of safflower seeds were identified.It was found that the expression of ACC, FATA, DGAT1 and FAD2 enzyme genes promoted the accumulation of fatty acids in safflower seeds.
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基本信息:
DOI：10.16445/j.cnki.1000-2340.20250407.005
中图分类号:S567.219
引用信息:
[1]刘旸,苌建峰,张博,等.红花种子脂肪酸代谢关键基因的挖掘与分析[J].河南农业大学学报,2026,60(02):217-227.DOI:10.16445/j.cnki.1000-2340.20250407.005.
基金信息:
国家自然科学基金青年基金项目（32300334）; 河南省高等学校青年骨干教师培养计划（2019GGJS045）; 2024年河南省中药材产业科技特派员服务团项目; 河南省中药材产业技术体系项目（HARS-22-11-G4）
2025-01-14
2025
2025-04-01
2025-04-01
2025
1
2025-04-08
2025-04-08
2025-04-08