| 496 | 0 | 364 |
| 下载次数 | 被引频次 | 阅读次数 |
通过分析扩展蛋白家族α-Expansin(EXPA)和β-Expansin(EXPB)的结构、蠕变特性,对其在植物生长发育和胁迫响应中的研究进展进行全面综述,探讨了EXPA与EXPB在种子萌发、籽粒发育、花粉管生长及胁迫响应中的关键作用。结合基因组学与蛋白质组学的最新成果,展望了扩展蛋白在作物抗逆性改良与产量提升中的应用潜力,以及其在作物改良和农业生产中的重要作用。
Abstract:By analyzing the structure and creep characteristics of the expansin family:α-Expansin(EXPA) and β-Expansin(EXPB), the research progress of EXPA and EXPB in plant growth and development and stress response was comprehensively reviewed. The key roles of EXPA and EXPB in seed germination, grain development, pollen tube growth and stress response were discussed. Combined with the latest achievements in genomics and proteomics, this review looks forward to the application potential of expansins in crop stress resistance improvement and yield improvement, as well as their importance in crop improvement and agricultural production.
[1] RAYLE D L, CLELAND R E. The acid growth theory of auxin-induced cell elongation is alive and well[J].Plant Physiology, 1992, 99(4):1271-1274.
[2] COSGROVE D J. Plant cell wall loosening by expansins[J]. Annual Review of Cell and Developmental Biology, 2024, 40(1):329-352.
[3] COSGROVE D J. Non-enzymatic action of expansins[J]. Journal of Biological Chemistry, 2020, 295(19):6782.
[4] SAMALOVA M, GAHUROVA E, HEJATKO J. Expansin-mediated developmental and adaptive responses:a matter of cell wall biomechanics[J]. Quantitative Plant Biology, 2022, 3:11.
[5] MCQUEEN-MASON S, DURACHKO D M, COSGROVE D J. Two endogenous proteins that induce cell wall extension in plants[J]. The Plant Cell, 1992, 4(11):1425-1433.
[6] COSGROVE D J. Structure and growth of plant cell walls[J]. Nature Reviews Molecular Cell Biology,2024, 25(5):340-358.
[7] SAMPEDRO J, GUTTMAN M, LI L C, et al. Evolutionary divergence of β-expansin structure and function in grasses parallels emergence of distinctive primary cell wall traits[J]. The Plant Journal, 2015, 81(1):108-120.
[8] HEPLER N K, BOWMAN A, CAREY R E, et al.Expansin gene loss is a common occurrence during adaptation to an aquatic environment[J]. The Plant Journal,2020, 101(3):666-680.
[9] COSGROVE D J. Expanding wheat yields with expansin[J]. New Phytologist, 2021, 230(2):403-405.
[10] DE SOUZA-NETO R R, DA COSTA VASCONCELOS F N, TEPER D, et al. The expansin gene CsLIEXP1 is a direct target of CsLOB1 in Citrus[J]. Phytopathology,2023, 113(7):1266-1277.
[11] K?K B?,CELIK ALTUNOGLU Y,?NCüL A B, et al. Expansin gene family database:a comprehensive bioinformatics resource for plant expansin multigene family[J]. Journal of Bioinformatics and Computational Biology, 2023, 21(3):2350015.
[12] LI M, LIU T, CAO R, et al. Evolution and expression of the expansin genes in emmer wheat[J]. International Journal of Molecular Sciences, 2023, 24(18):14120.
[13] SUN Q, LI Y F, GONG D M, et al. A NACEXPANSIN module enhances maize kernel size by controlling nucellus elimination[J]. Nature Communications, 2022, 13(1):5708.
[14]付涵芬. Ruminococcus flavefaciens扩展蛋白Y189的结构域改造及其性质表征[D].郑州:河南农业大学,2024.FU H F. Modification of domains and properties characterizationof the expansin-like protein Y189 from Ruminococcus flavefaciens[D]. Zhengzhou:Henan Agricultural University, 2024.
[15] FARIS A D, CELIK A Y, HORUZ E, et al. Investigation of the expansin gene family in sugar beet(Beta vulgaris)by the genome-wide level and their expression responses under abiotic stresses[J]. Biologia Futura,2023, 74(3):295-307.
[16] SU G Q, LIN Y F, WANG C F, et al. Expansin SlExp1and endoglucanase SlCel2 synergistically promote fruit softening and cell wall disassembly in tomato[J]. The Plant Cell, 2024, 36(3):709-726.
[17] ZHANG B Y, CHANG L, SUN W N, et al. Overexpression of an expansin-like gene, GhEXLB2 enhanced drought tolerance in cotton[J]. Plant Physiology and Biochemistry, 2021, 162:468-475.
[18] DU M M, SPALDING E P, GRAY W M. Rapid auxinmediated cell expansion[J]. Annual Review of Plant Biology, 2020, 71:379-402.
[19] LIN W W, ZHOU X, TANG W X, et al. TMK-based cell-surface auxin signalling activates cell-wall acidification[J]. Nature, 2021, 599(7884):278-282.
[20] LI L X, VERSTRAETEN I, ROOSJEN M, et al. Cell surface and intracellular auxin signalling for H+fluxes in root growth[J]. Nature, 2021, 599(7884):273-277.
[21] POLAK M, KARCZ W. Some new methodological and conceptual aspects of the“acid growth theory” for the auxin action in maize(Zea mays L.)coleoptile segments:do acid-and auxin-induced rapid growth differ in their mechanisms[J]. International Journal of Molecular Sciences, 2021, 22(5):2317.
[22] MICHALAK A, WDOWIKOWSKA A, JANICKA M.Plant plasma membrane proton pump:one protein with multiple functions[J]. Cells, 2022, 11(24):4052.
[23] BALL T, EDSTROM W, MAUCH L, et al. Gain of structure and IgE epitopes by eukaryotic expression of the major Timothy grass pollen allergen, Phl p 1[J].The FEBS Journal, 2005, 272(1):217-227.
[24] SEADER V H, THORNSBERRY J M, CAREY R E.Utility of the Amborella trichopoda expansin superfamily in elucidating the history of angiosperm expansins[J].Journal of Plant Research, 2016, 129(2):199-207.
[25]姜志磊,周琳,武奉慈,等.玉米扩展蛋白Expansin基因家族定位及基因表达模式分析[J].生物技术进展,2018, 8(1):34-40.JIANG Z L, ZHOU L, WU F C, et al. Analysis of location and expression pattern of maize expansin gene family[J]. Current Biotechnology, 2018, 8(1):34-40.
[26] HAN Z, LIU Y, DENG X, et al. Genome-wide identification and expression analysis of expansin gene family in common wheat(Triticum aestivum L.)[J]. BMC Genomics, 2019, 20(1):101.
[27] YIN Z H, ZHOU F W, CHEN Y N, et al. Genomewide analysis of the expansin gene family in Populus and characterization of expression changes in response to phytohormone(abscisic acid)and abiotic(lowtemperature)stresses[J]. International Journal of Molecular Sciences, 2023, 24(9):7759.
[28]李娜,张华,邢馨竹,等.大豆扩展蛋白基因GmEXLA1在荚果发育中的功能鉴定[J].中国农业科技导报,2025, 27(3):49-59.LI N, ZHANG H, XING X Z, et al. Function analysis of soybean expansin gene GmEXLA1 in plant pod and seed development[J]. Journal of Agricultural Science and Technology, 2025, 27(3):49-59.
[29]徐筱,徐倩,张磖,等.植物扩展蛋白基因的研究进展[J].北京林业大学学报,2010, 32(5):154-162.XU X, XU Q, ZHANG L, et al. Advancements in expansin genes of plants[J]. Journal of Beijing Forestry University, 2010, 32(5):154-162.
[30]李必聪,李慧英,肖遥,等.芋扩展蛋白基因家族的全基因组鉴定及其在球茎膨大中的表达分析[J].浙江农业学报,2023, 35(7):1604-1616.LI B C, LI H Y, XIAO Y, et al. Genome-wide identification and expression analysis of expansin gene family in corm expan-Sion of Colocasia esculenta[J]. Acta Agriculturae Zhejiangensis, 2023, 35(7):1604-1616.
[31]马霜,王博雅,曹颖,等.毛竹扩展蛋白基因的鉴定及其表达分析[J].植物研究,2022, 42(1):29-38.MA S, WANG B Y, CAO Y, et al. Identification and expression analysis of expansin genes in moso bamboo(Phyllostachys edulis)[J]. Bulletin of Botanical Research, 2022, 42(1):29-38.
[32] ZENG H Y, DENG S Y, JIN C C, et al. Origin and evolution of auxin-mediated acid growth[J]. Proceedings of the National Academy of Sciences of the United States of America, 2024, 121(51):e2412493121.
[33] ZHANG T, TANG H S, VAVYLONIS D, et al. Disentangling loosening from softening:insights into primary cell wall structure[J]. The Plant Journal, 2019, 100(6):1101-1117.
[34] ISHIDA K, YOKOYAMA R. Reconsidering the function of the xyloglucan endotransglucosylase/hydrolase family[J]. Journal of Plant Research, 2022, 135(2):145-156.
[35] PARK Y B, COSGROVE D J. A revised architecture of primary cell walls based on biomechanical changes induced by substrate-specific endoglucanases[J]. Plant Physiology, 2012, 158(4):1933-1943.
[36] YU J Y, ZHANG Y, COSGROVE D J. The nonlinear mechanics of highly extensible plant epidermal cell walls[J]. Proceedings of the National Academy of Sciences of the United States of America, 2024, 121(2):e2316396121.
[37] TABUCHI A, LI L C, COSGROVE D J. Matrix solubilization and cell wall weakening by β-expansin(group-1allergen)from maize pollen[J]. The Plant Journal,2011, 68(3):546-559.
[38] MCQUEEN-MASON S J, COSGROVE D J. Expansin mode of action on cell walls[J]. Plant Physiology,1995, 107(1):87-100.
[39] WANG T, PARK Y B, CAPORINI M A, et al.Sensitivity-enhanced solid-state NMR detection of expansin’s target in plant cell walls[J]. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(41):16444-16449.
[40] CHANLIAUD E, BURROWS K M, JERONIMIDIS G,et al. Mechanical properties of primary plant cell wall analogues[J]. Planta, 2002, 215(6):989-996.
[41] YENNAWAR N H, LI L C, DUDZINSKI D M, et al.Crystal structure and activities of EXPB1(Zea m 1), a beta-expansin and group-1 pollen allergen from maize[J]. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103(40):14664-14671.
[42] WANG T, CHEN Y N, TABUCHI A, et al. The target of β-expansin EXPB1 in maize cell walls from binding and solid-state NMR studies[J]. Plant Physiology,2016, 172(4):2107-2119.
[43] HEPLER N K, COSGROVE D J. Directed in vitro evolution of bacterial expansin BsEXLX1 for higher cellulose binding and its consequences for plant cell wallloosening activities[J]. FEBS Letters, 2019, 593(18):2545-2555.
[44] LIN C F, CHOI H S, CHO H T. Root hair-specific EXPANSIN A7 is required for root hair elongation in Arabidopsis[J]. Molecules and Cells, 2011, 31(4):393-397.
[45] LüP T, KANG M, JIANG X Q, et al. RhEXPA4, a rose expansin gene, modulates leaf growth and confers drought and salt tolerance to Arabidopsis[J]. Planta,2013, 237(6):1547-1559.
[46] MAROWA P, DING A M, KONG Y Z. Expansins:roles in plant growth and potential applications in crop improvement[J]. Plant Cell Reports, 2016, 35(5):949-965.
[47]陈兵先,张琪,戴彰言,等.水杨酸引发提高低温下水稻种子萌发活力的生理与分子效应[J].中国农业科学,2024, 57(7):1220-1236.CHEN B X, ZHANG Q, DAI Z Y, et al. Physiological and molecular effects of salicylic acid on rice seed germination at low temperature[J]. Scientia Agricultura Sinica, 2024, 57(7):1220-1236.
[48] FENG X, LI C T, HE F M, et al. Genome-wide identification of expansin genes in wild soybean(Glycine soja)and functional characterization of Expansin B1(GsEXPB1)in soybean hair root[J]. International Journal of Molecular Sciences, 2022, 23(10):5407.
[49] CALDERINI D F, CASTILLO F M, ARENAS-M A, et al. Overcoming the trade-off between grain weight and number in wheat by the ectopic expression of expansin in developing seeds leads to increased yield potential[J]. New Phytologist, 2021, 230(2):629-640.
[50] MIRA J P, ARENAS-M A, CALDERINI D F, et al.Integrated transcriptome analysis identified key expansin genes associated with wheat cell wall, grain weight and yield[J]. Plants, 2023, 12(15):2868.
[51] GONG D M, TAN Z D, ZHAO H L, et al. Fine mapping of a kernel length-related gene with potential value for maize breeding[J]. Theoretical and Applied Genetics, 2021, 134(4):1033-1045.
[52] LIU C Y, ZHAO H Y, LI J Y, et al. Identification of candidate expansin genes associated with seed weight in pomegranate(Punica granatum L.)[J]. Genes, 2024,15(2):212.
[53] VALDIVIA E R, SAMPEDRO J, LAMB J C, et al.Recent proliferation and translocation of pollen group 1allergen genes in the maize genome[J]. Plant Physiology, 2007, 143(3):1269-1281.
[54] LI L C, BEDINGER P A, VOLK C, et al. Purification and characterization of four β-expansins(Zea m 1 isoforms)from maize pollen[J]. Plant Physiology, 2003,132(4):2073-2085.
[55] VALDIVIA E R, STEPHENSON A G, DURACHKO D M, et al. Class B beta-expansins are needed for pollen separation and stigma penetration[J]. Sexual Plant Reproduction, 2009, 22(3):141-152.
[56] GENG X X, WANG X X, WANG J C, et al. TaEXPB5functions as a gene related to pollen development in thermo-sensitive male-sterility wheat with Aegilops kotschyi cytoplasm[J]. Plant Science, 2022, 323:111377.
[57] LIU W M, XU L A, LIN H, et al. Two expansin genes,AtEXPA4 and AtEXPB5, are redundantly required for pollen tube growth and AtEXPA4 is involved in primary root elongation in Arabidopsis thaliana[J]. Genes,2021, 12(2):249.
[58] WANG Y C, LIU P P, CAI Y L, et al. PbrBZR1 interacts with PbrARI2. 3 to mediate brassinosteroidregulated pollen tube growth during self-incompatibility signaling in pear[J]. Plant Physiology, 2023, 192(3):2356-2373.
[59]王亚玲,闫鹏涛,张丽娟,等.环核苷酸门控离子通道蛋白在植物生长发育及胁迫响应中的研究进展[J].河南农业大学学报,2025, 59(1):21-28.WANG Y L, YAN P T, ZHANG L J, et al. Research progress on cyclic nucleotide gated ion channel proteins in plant growth, development and stress response[J].Journal of Henan Agricultural University, 2025, 59(1):21-28.
[60] WU Y, THORNE E T, SHARP R E, et al. Modification of expansin transcript levels in the maize primary root at low water potentials[J]. Plant Physiology,2001, 126(4):1471-1479.
[61]李佳欣,孙莹莹,邓雅文,等.大豆GmEXPB3和GmEXPB6生物信息学分析及干旱和盐胁迫诱导表达[J].大豆科学,2024, 43(5):559-564.LI J X, SUN Y Y, DENG Y W, et al. Bioinformatics analysis of GmEXPB3 and GmEXPB6 and their expression induced by drought and salt stress in soybean[J].Soybean Science, 2024, 43(5):559-564.
[62] LIU B X, ZHANG B, YANG Z R, et al. Manipulating ZmEXPA4 expression ameliorates the drought-induced prolonged anthesis and silking interval in maize[J].The Plant Cell, 2021, 33(6):2058-2071.
[63] TAO K Y, LI Y, HU Y, et al. Overexpression of ZmEXPA5 reduces anthesis-silking interval and increases grain yield under drought and well-watered conditions in maize[J]. Molecular Breeding, 2023, 43(12):84.
[64] YANG J J, ZHANG G Q, AN J, et al. Expansin gene TaEXPA2 positively regulates drought tolerance in transgenic wheat(Triticum aestivum L.)[J]. Plant Science,2020, 298:110596.
[65]李雪芳,柳雪,王西娜,等.盐胁迫对嫁接西瓜幼苗根系构型、活力及离子分配的影响[J].南方农业学报,2024, 55(10):3084-3095.LI X F, LIU X, WANG X N, et al. Effects of salt stress on root configuration, vitality and ion distribution in grafted watermelon seedlings[J]. Journal of Southern Agriculture, 2024, 55(10):3084-3095.
[66] WANG X, MA J, HE F M, et al. A study on the functional identification of overexpressing winter wheat expansin gene TaEXPA7-B in rice under salt stress[J].International Journal of Molecular Sciences, 2024, 25(14):7707.
[67]王玉斌,张彦威,刘薇,等.大豆盐胁迫响应扩展蛋白基因的筛选及GmEXPA17a的克隆和表达分析[J].山东农业科学,2022, 54(11):11-18.WANG Y B, ZHANG Y W, LIU W, et al. Screening of soybean expansins genes response to salt stress and cloning and expression analysis of GmEXPA17a[J]. Shandong Agricultural Sciences, 2022, 54(11):11-18.
[68] WANG L L, ZHANG T, LI C T, et al. Overexpression of wild soybean expansin gene GsEXLB14 enhanced the tolerance of transgenic soybean hairy roots to salt and drought stresses[J]. Plants, 2024, 13(12):1656.
[69] LIU Z, YIN K X, ZHANG Y, et al. Populus trichocarpa EXPA6 facilitates radial and longitudinal transport of Na+under salt stress[J]. International Journal of Molecular Sciences, 2024, 25(17):9354.
[70] LIU Q K, LI P T, UMER M J, et al. Identification of EXPA4 as a key gene in cotton salt stress adaptation through transcriptomic and coexpression network analysis of root tip protoplasts[J]. BMC Plant Biology,2025, 25(1):65.
[71] SUN W J, YAO M, WANG Z, et al. Involvement of auxin-mediated CqEXPA50 contributes to salt tolerance in quinoa(Chenopodium quinoa)by interaction with auxin pathway genes[J]. International Journal of Molecular Sciences, 2022, 23(15):8480.
[72] ZHANG J K, WANG L, ZHAO H, et al. The SmWRKY12-SmRAP2-7-SmEXPA13 module in Salix matsudana koidz enhances plant tolerance to drought stress[J]. International Journal of Biological Macromolecules, 2025, 284(Pt 1):138077.
[73] REFAIY M, TAHIR M, JIAO L J, et al. Genome-wide identification of xyloglucan endotransglucosylase/hydrolase multigene family in Chinese jujube(Ziziphus jujuba)and their expression patterns under different environmental stresses[J]. Plants, 2024, 13(24):3503.
基本信息:
DOI:10.16445/j.cnki.1000-2340.20250317.001
中图分类号:S311
引用信息:
[1]张丽娟,左传顺,高可晓,等.扩展蛋白EXPA和EXPB在植物生长发育及胁迫响应中的研究进展[J].河南农业大学学报,2025,59(05):776-784.DOI:10.16445/j.cnki.1000-2340.20250317.001.
基金信息:
国家自然科学基金青年基金项目(31800260); 河南省科技攻关项目(252102111117); 河南农业大学青年英才基金项目(30501043)