| 648 | 1 | 102 |
| 下载次数 | 被引频次 | 阅读次数 |
【目的】研究白藜芦醇(resveratrol, RES)对鼠伤寒沙门氏菌(Salmonella typhimurium)诱导猪小肠上皮细胞(intestinal porcine epithelial cells, IPEC-J2)损伤的缓解作用，为促进动物健康养殖提供新思路。【方法】用鼠伤寒沙门氏菌建立IPEC-J2损伤模型，测定细胞上清液一氧化氮(NO)浓度、肿瘤坏死因子-α(TNF-α)质量浓度、白介素-6(IL-6)质量浓度、白介素-4(IL-4)质量浓度、乳酸脱氢酶(lactate dehydrogenase, LDH)水平、总超氧化物歧化酶(total superoxide dismutase, T-SOD)活力、还原型谷胱甘肽(glutathione, GSH)质量浓度和丙二醛(malondialdehyde, MDA)浓度，分析RES对IPEC-J2损伤模型的缓解作用。【结果】RES预处理IPEC-J2细胞的最佳质量浓度为7.8 mg·L-1,且能有效缓解鼠伤寒沙门氏菌诱导IPEC-J2细胞产生的炎症损伤，显著降低促炎因子(TNF-α和IL-6)的质量浓度，提高抑炎因子(IL-4)的表达，进而有效减缓肠道炎症反应。同时，RES可显著提高T-SOD活力和GSH质量浓度，显著降低MDA浓度，缓解鼠伤寒沙门氏菌诱发的氧化损伤，达到维持机体肠道氧化还原平衡的稳态。【结论】RES对IPEC-J2损伤具有保护作用，可用于预防鼠伤寒沙门氏菌感染。
Abstract：【Objective】To investigate the protective effects of resveratrol(RES) against oxidative and inflammatory damage induced by Salmonella typhimurium in intestinal porcine epithelial(IPEC-J2) cells and provide insights to enhance animal health in breeding practices.【Method】An IPEC-J2 cell injury model was established using S.typhimurium, and key oxidative stress and inflammatory indicators were measured in the cell supernatant, including nitric oxide(NO) content, tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), interleukin-4(IL-4) secretion, lactate dehydrogenase(LDH) level, total superoxide dismutase(T-SOD), reduced glutathione(GSH) and malondialdehyde(MDA), to analyze the mitigating effects of RES on IPEC-J2 cell injury model. 【Result】Pretreatment of IPEC-J2 cells with RES at an optimal mass concentration of 7.8 mg·L-1 could significantly alleviate the inflammatory injury induced by S.typhimurium, notably reducing the contents of pro-inflammatory factors(TNF-α and IL-6), and increasing the expression of inhibitory factors(IL-4), thus effectively slowing down the intestinal inflammatory response. Meanwhile, RES could significantly increase the activities of T-SOD and GSH enzymes, significantly reduce the expression level of MDA, and alleviate the oxidative damage induced by S.typhimurium, thereby contributing to the maintenance of intestinal redox homeostasis of the organism. 【Conclusion】RES has a protective effect on IPEC-J2 cell damage and holds potential for preventing S.typhimurium infection in animal models.
[1] 王娜，高恩光，李娜，等.白藜芦醇与维生素E协同抗氧化效应研究[J].河南农业大学学报，2022,56(6):1007-1014.WANG N,GAO E G,LI N,et al.Study on the synergistic antioxidant effect of resveratrol and vitamin E[J].Journal of Henan Agricultural University,2022,56(6):1007-1014.
[2] 王倩，杨豹，李银，等.白藜芦醇通过ESR1-PI3K信号通路抑制3T3-L1细胞脂滴形成[J].南方农业学报，2024,55(7):1916-1924.WANG Q,YANG B,LI Y,et al.Resveratrol inhibits lipid droplet formation in 3T3-L1 cells via ESR1-PI3K signaling pathway[J].Journal of Southern Agriculture,2024,55(7):1916-1924.
[3] PENG W,HENGFAN N I,GUO D L,et al.Farnesoid X receptor regulators from natural products and their biological function[J].Journal of Traditional Chinese Medicine,2023,43(3):618-626.
[4] MENG Q W,LI J W,WANG C S,et al.Biological function of resveratrol and its application in animal production:a review[J].Journal of Animal Science and Biotechnology,2023,14(1):25.
[5] CUI Q K,FU Q T,ZHAO X H,et al.Protective effects and immunomodulation on piglets infected with rotavirus following resveratrol supplementation[J].PLoS One,2018,13(2):e0192692.
[6] 符清瑶，曹婷，施力光，等.白藜芦醇的生物活性与其在畜牧生产中的应用进展[J].家畜生态学报，2021,42(6):79-84.FU Q Y,CAO T,SHI L G,et al.Biological activities of resveratrol and its application progress in animal husbandry[J].Journal of Domestic Animal Ecology,2021,42(6):79-84.
[7] YU S,ZOU L P,ZHAO J W,et al.Resveratrol alleviates fumonisin-induced intestinal cytotoxicity by modulating apoptosis,tight junction,and inflammation in IPEC-J2 porcine intestinal epithelial cells[J].Environmental Toxicology,2024,39(2):905-914.
[8] 王娜，宁灿灿，赵峥，等.花生芽白藜芦醇大孔树脂纯化工艺及抑菌功效[J].农业工程学报，2022,38(18):293-301.WANG N,NING C C,ZHAO Z,et al.Purification process by macroporous resin and antibacterial efficacy of resveratrol from peanut buds[J].Transactions of the Chinese Society of Agricultural Engineering,2022,38(18):293-301.
[9] EBANI V V,NARDONI S,BERTELLONI F,et al.In vitro antimicrobial activity of essential oils against Salmonella enterica serotypes enteritidis and typhimurium strains isolated from poultry[J].Molecules,2019,24(5):900.
[10] HEREDIA N,GARCíA S.Animals as sources of food-borne pathogens:a review[J].Animal Nutrition,2018,4(3):250-255.
[11] YANG J,ZHU C,YE J L,et al.Protection of porcine intestinal-epithelial cells from deoxynivalenol-induced damage by resveratrol via the Nrf2 signaling pathway[J].Journal of Agricultural and Food Chemistry,2019,67(6):1726-1735.
[12] LUO X,WU S Z,JIA H,et al.Resveratrol alleviates enterotoxigenic Escherichia coli K88-induced damage by regulating SIRT-1 signaling in intestinal porcine epithelial cells[J].Food & Function,2022,13(13):7346-7360.
[13] 尚智援，窦彩霞，王克玮，等.丁酸梭菌通过激活p38丝裂原活化蛋白激酶/核因子E2相关因子信号通路减轻猪霍乱沙门氏菌导致的猪小肠上皮细胞氧化损伤[J].动物营养学报，2021,33(12):7105-7117.SHANG Z Y,DOU C X,WANG K W,et al.Clostridium butyricum alleviates porcine intestinal epithelial cells oxidative damage induced by Salmonella cholerae through activating p38 mitogen activated protein Kinase/Nuclear factor E2 related factor signaling pathway[J].Chinese Journal of Animal Nutrition,2021,33(12):7105-7117.
[14] 何传波，邓婷，魏好程，等.仙草多糖对细胞氧化损伤的保护作用[J].食品科学，2020,41(13):160-168.HE C B,DENG T,WEI H C,et al.Protective effect of polysaccharide from Mesona blumes on oxidative damage of cells[J].Food Science,2020,41(13):160-168.
[15] 王怡梦，刘雪姣，王倩，等.沙门菌通过NF-κB/β-catenin信号通路引致IPEC-J2损伤的分子机制[J].畜牧兽医学报，2021,52(1):227-237.WANG Y M,LIU X J,WANG Q,et al.Molecular mechanism of the injury of IPEC-J2 caused by Salmonella via NF-κB/β-catenin signaling pathway[J].Acta Veterinaria et Zootechnica Sinica,2021,52(1):227-237.
[16] ZHUANG Y,WU H R,WANG X X,et al.Resveratrol attenuates oxidative stress-induced intestinal barrier injury through PI3K/akt-mediated Nrf2 signaling pathway[J].Oxidative Medicine and Cellular Longevity,2019,2019:7591840.
[17] UPADHAYA S D,KIM I H.Maintenance of gut microbiome stability for optimum intestinal health in pigs-a review[J].Journal of Animal Science and Biotechnology,2022,13(1):140.
[18] 李静，武万强，杨靖亚.紫草素可抑制在RAW264.7巨噬细胞和斑马鱼幼虫中由脂多糖诱导的炎症反应[J].甘肃农业大学学报，2023,58(1):38-45.LI J,WU W Q,YANG J Y.Shikonin inhibits lipopolysaccharide-induced inflammatory responses in RAW264.7 macrophages and zebrafish larvae[J].Journal of Gansu Agricultural University,2023,58(1):38-45.
[19] ZHAO L,LIU X D,GOMEZ N A,et al.Stage-specific nutritional management and developmental programming to optimize meat production[J].Journal of Animal Science and Biotechnology,2023,14(1):2.
[20] 李孔会，廖森泰，李倩，等.山药多酚对结肠炎小鼠肠黏膜损伤预防作用研究[J].食品科学技术学报，2021,39(4):46-54.LI K H,LIAO S T,LI Q,et al.Study on preventive effect of Chinese yam peel polyphenols on intestinal mucosal injury in colitis mice[J].Journal of Food Science and Technology,2021,39(4):46-54.
[21] 全帅，吕开原，赵艳敏，等.葡萄籽提取物与柳氮磺吡啶联合用药对实验性溃疡性结肠炎的作用[J].药物评价研究，2021,44(3):478-486.QUAN S,Lü K Y,ZHAO Y M,et al.Study on combined treatment of grape seed extract and sulfasalazine for experimental ulcerative colitis[J].Drug Evaluation Research,2021,44(3):478-486.
[22] 张卫辉，朱秋凤，张永静，等.槲皮素对仔猪生长性能、肠道抗氧化及免疫相关基因表达的影响[J].河南农业大学学报，2023,57(1):136-142.ZHANG W H,ZHU Q F,ZHANG Y J,et al.Effects of quercetin on growth performance and intestinal antioxidant capacity and immune related gene expression in piglets[J].Journal of Henan Agricultural University,2023,57(1):136-142.
[23] 金钺，李滨洲，郭珍珍，等.猪源粪肠球菌对猪小肠上皮细胞黏附及致炎作用的影响[J].河南农业大学学报，2023,57(4):623-631.JIN Y,LI B Z,GUO Z Z,et al.Adhesion and inflammatory effect of Enterococcus faecalis to intestinal porcine epithelial cells[J].Journal of Henan Agricultural University,2023,57(4):623-631.
[24] CHI X X,HUANG M Z,TU H L,et al.Innate and adaptive immune abnormalities underlying autoimmune diseases:the genetic connections[J].Science China Life Sciences,2023,66(7):1482-1517.
[25] 赵雪芹.抗菌肽类似物JH-3抵御沙门氏菌感染的作用机制研究[D].新乡：河南科技学院，2018.ZHAO X Q.Mechanism of antimicrobial peptide analogue JH-3 against Salmonella[D].Xinxiang:Henan Institute of Science and Technology,2018.
[26] 司晓丽.芍药苷对结肠炎相关性结直肠癌的化学预防作用及机制研究[D].兰州：兰州大学，2022.SI X L.The chemoprevention and mechanism of paeoniflorin on colitis-associated colorectal cancer[D].Lanzhou:Lanzhou University,2022.
[27] 蔡茜茜，李晨，陈旭，等.笛鲷鱼鳞源功能多肽对Caco-2细胞氧化应激损伤的保护作用[J].食品科学，2023,44(7):10-17.CAI X X,LI C,CHEN X,et al.Protective effect of functional peptides derived from crimson snapper scales on oxidative stress damage in caco-2 cells[J].Food Science,2023,44(7):10-17.
[28] 崔海燕，纪龙翔，朱宇晴，等.黄芪多糖对脂多糖诱导肠上皮细胞IPEC-J2氧化应激和炎症反应的缓解作用[J].河南师范大学学报(自然科学版),2022,50(4):101-106.CUI H Y,JI L X,ZHU Y Q,et al.Astragalus polysaccharides alleviate lipopolysaccharide-induced oxidative stress and inflammation in IPEC-J2 cells[J].Journal of Henan Normal University (Natural Science Edition),2022,50(4):101-106.
[29] 陈敏，周金凤，韦崇万，等.白藜芦醇和EGCG联用对小鼠骨骼肌抗氧化能力的影响[J].南方农业学报，2022,53(9):2683-2690.CHEN M,ZHOU J F,WEI C W,et al.Effects of resveratrol and EGCG combination on antioxidant capacity of mouse skeletal muscle[J].Journal of Southern Agriculture,2022,53(9):2683-2690.
[30] 巫永华，张建萍，赵节昌，等.大孔树脂纯化黄精多酚及其抗氧化性与组成分析[J].农业工程学报，2020,36(1):318-326.WU Y H,ZHANG J P,ZHAO J C,et al.Antioxidantion and composition analysis of purified Polygonatum sibiricum polyphenols using macroporous resin[J].Transactions of the Chinese Society of Agricultural Engineering,2020,36(1):318-326.
基本信息:
DOI：10.16445/j.cnki.1000-2340.20240328.001
中图分类号:S853.74
引用信息:
[1]赵峥,余秋颖,杨聪燕等.白藜芦醇对鼠伤寒沙门氏菌诱导型IPEC-J2细胞损伤的缓解作用[J].河南农业大学学报,2024,58(06):957-965.DOI:10.16445/j.cnki.1000-2340.20240328.001.
基金信息:
河南省重大科技专项(221100110600); 河南省创新生态支撑专项(HARS-22-05-Z1)