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【目的】创建小麦TaMBD9基因敲除材料，探讨该基因在小麦生长发育调控过程中的生物学功能。【方法】利用实时定量反转录聚合酶链反应（quantitative reverse transcription polymerase chain reaction, qRT-PCR）分析TaMBD9的表达特性，通过CRISPR-Cas9介导的基因编辑技术创建基因敲除小麦材料。【结果】表达分析显示，TaMBD9在小麦不同组织间存在差异表达，其中叶片中的表达量最高，且随发育进程有所波动。3个部分同源基因间表达水平存在一定差异，其中TaMBD9D的表达水平较高。获得了TaMBD9的3个部分同源基因均成功敲除的纯合突变小麦材料。表型分析发现，TaMBD9敲除导致小麦叶夹角明显增大。【结论】小麦TaMBD9在叶夹角调控过程中发挥重要功能。研究获得了TaMBD9基因敲除纯合株系，为开展小麦叶夹角分子调控机制研究提供了新材料。
Abstract：【Objective】To obtain gene knockout materials of TaMBD9 and explore its biological function in the regulation of growth and development in wheat. 【Method】The expression pattern of TaMBD9was analyzed by qRT-PCR and the gene knockout wheat materials were created by CRISPR-Cas9-mediated gene editing technology. 【Result】The expression analysis showed that TaMBD9 was differentially expressed among wheat tissues with the highest expression level in leaves, and its expression in leaves fluctuated with the development process. There were some differences in the expression levels among three homoeologs, and the expression level of TaMBD9D was higher than that of the other two homoeologs in detected tissues. The homozygous wheat mutants were obtained by sucessfully knocking out all three homoeologs of TaMBD9. Phenotypic analysis demonstrated that the knockout of TaMBD9 resulted in a significant increase of leaf angle in wheat. 【Conclusion】The TaMBD9 plays an important role in the regulation of leaf angle in wheat, and the obtained gene knockout lines of TaMBD9 provides new materials for further exploring the molecular regulatory mechanisms of leaf angle in wheat.
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基本信息:
DOI：10.16445/j.cnki.1000-2340.20240517.001
中图分类号:S512.1
引用信息:
[1]范姗姗,杜金强,张新宁等.小麦TaMBD9基因敲除及其对叶夹角的影响[J].河南农业大学学报,2025,59(01):29-37.DOI:10.16445/j.cnki.1000-2340.20240517.001.
基金信息:
国家重点研发计划项目（2017YFD0301100）; 中原学者工作站项目（234400510009）