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2024 03 v.58;No.243 394-402
基于全基因组SNPs标记对河南斗鸡遗传多样性及选择信号分析
基金项目(Foundation): 神农种业实验室一流课题(SN01-2022-05);; 河南省优秀青年科学基金项目(232300421034)
邮箱(Email):
DOI: 10.16445/j.cnki.1000-2340.20240328.002
中文作者单位:

神农种业实验室;河南农业大学动物科技学院;

摘要(Abstract):

【目的】对河南斗鸡品种的遗传多样性与全基因组选择信号进行分析,挖掘河南斗鸡品种重要的种质特性基因。【方法】使用AffymetrixAxiom 600K高密度鸡基因分型芯片对来自9个品种的173只鸡的群体(包括20只河南斗鸡及153只商品鸡)进行基因分型;计算各个品种的期望杂合度、观测杂合度、次等位基因频率及核苷酸多样性评估地方鸡群体的遗传多样性;通过构建系统发育树、主成分分析、祖先成分分析方法研究品种的群体结构;利用斗鸡与商品鸡的成对遗传分化指数值进行选择信号分析。【结果】河南斗鸡及各商品鸡群体的观测杂合度为0.153~0.311,期望杂合度为0.158~0.315,次等位基因频率为0.111~0.234,核苷酸多样性为9.77×10-5~1.56×10-4,且斗鸡的遗传多样性低于商品肉鸡品种,高于商品蛋鸡品种。系统发育树、主成分分析及祖先成分分析表明品种间有明显的群体分化。河南斗鸡与商品鸡群的主成分分析发现,河南斗鸡与商品肉鸡品种的遗传距离相对较近;将河南斗鸡和商品鸡群进行遗传选择信号后分析发现,河南斗鸡在神经,骨骼肌肉发育,免疫等性状经过高度选择。【结论】本研究从全基因组水平探究了河南斗鸡的遗传多样性和群体结构,筛选出候选基因,为河南斗鸡遗传资源保护和利用提供参考。

关键词(KeyWords): 河南斗鸡;;遗传多样性;;群体结构;;选择信号;;全基因组;;单核苷酸多态性
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基本信息:

DOI:10.16445/j.cnki.1000-2340.20240328.002

中图分类号:S831.2

引用信息:

[1]胡晓玉,肖成朋,高超群等.基于全基因组SNPs标记对河南斗鸡遗传多样性及选择信号分析[J].河南农业大学学报,2024,58(03):394-402.DOI:10.16445/j.cnki.1000-2340.20240328.002.

基金信息:

神农种业实验室一流课题(SN01-2022-05);; 河南省优秀青年科学基金项目(232300421034)

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