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采用孔径为0.2μm的微滤膜、0.004μm的超滤膜(5 000 D)和0.001μm的纳滤膜(150 D)及反渗透膜等4种不同卷式膜元件对地黄根区土壤水提液中的化感物质进行富集,测定了富集效率、不同截留液中的酚酸类化感物质含量等,并通过盆栽试验对不同截留液的化感效应进行了验证。结果表明,纳滤膜对根区土壤水提液的富集效率最高,达到3.607 mg·kg-1;HPLC也表明,纳滤膜截留液中酚酸类化感物质的种类和含量最多。盆栽试验结果表明,与对照相比,添加了纳滤膜截留液的盆栽地黄连作障碍效应最明显,地上地下部鲜质量分别为1.29,4.57 g,根系投影面积为47.489 cm2,平均体积为0.754 cm3,添加了微滤膜截留液的地黄连作障碍效应也比较明显,而添加了超滤膜和反渗透膜截留液的盆栽地黄生长状况良好,与富集效率及HPLC分析结果相一致。
Abstract：The enrichment of soil allelochemicals in root zone is one of the difficulties in the study of continuous cropping obstacles of Rehmannia glutinosa. Four kinds of membrane elements with microfiltration membrane with pore size of 0.2 μm, ultrafiltration membrane with pore size of 0.004 μm(5 000 D), nanofiltration membrane with pore size of 0.001 μm(150 D) and reverse osmosis membrane were used to enrich the allelochemicals in the soil aqueous extracts of Rehmannia glutinosa. The enrichment efficiency and the content of phenolic acid allelopathic substances in different intercepting fluids were determined. And the allelopathic effects of different intercepting fluids were verified by pot experiments. The results showed that the enrichment efficiency of the nanofiltration membrane was the highest, reaching 3.607 mg· kg-1. HPLC analysis also showed that the types and contents of phenolic acid allelopathic substances in the nanofiltration membrane intercepting fluid were the largest. Compared with the control, the results of pot experiment showed that the obstacles effect of continuous cropping of Rehmannia glutinosa was the most obvious with nanofiltration membrane. The fresh weight of above ground and underground parts were 1.29 g and 4.57 g, the root projected area was 47.489 cm2, and the average volume was 0.754 cm3. The continuous cropping effect of the Rehmannia glutinosa with the microfiltration membrane retentate was also obvious, while the growth of potted Rehmannia glutinosa with the ultrafiltration membrane and reverse osmosis membrane retentate were good, which was consistent with the enrichment efficiency and HPLC analysis results. The roll membrane technology can be applied to the enrichment of soil allelochemicals in the root zone of Rehmannia glutinosa. The method is convenient and quick, and can improve the extraction efficiency of allelochemicals.
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基本信息:
DOI：10.16445/j.cnki.1000-2340.20210122.008
中图分类号:S567.234
引用信息:
[1]孙香荣,李娟,钮颜宇,等.基于膜技术的地黄根区土壤化感物质分离效果研究[J].河南农业大学学报,2021,55(01):35-43.DOI:10.16445/j.cnki.1000-2340.20210122.008.
基金信息:
国家自然科学基金资助项目(81373910,81102756)
2021-01-22
2021-01-22
2021-01-22