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【目的】探究施用高碳基土壤修复肥对烤烟根际土壤细菌群落结构及烟叶产值的影响。【方法】采用田间试验方式，分别设置高碳基土壤修复肥0 kg·hm-2(CK)和600 kg·hm-2(T)2组处理。【结果】烟株移栽90 d后，T处理显著提高了土壤有机质含量(23.59%)、碱解氮含量(17.41%)、pH值(1.07个单位)和含水率(24.17%);显著提高了土壤蔗糖酶活性(97.65%)、中性磷酸酶活性(363.64%)、脲酶活性(356.60%)和过氧化氢酶活性(19.02%);显著增加了细菌Chao指数、Observed＿OTUS和Shannon指数，降低了Simpson指数。其中GAL15菌门、芽单胞菌门和硝化螺旋菌门相对丰度显著减少；奇古菌门相对丰度显著增加；显著提升了烟叶经济性状，上等烟比例和产值分别提高12.74%和12.15%。【结论】施加高碳基土壤修复肥改变了土壤细菌群落结构，增加了土壤养分含量及酶活性，改善土壤pH值，提高了烤后烟叶品质。
Abstract：【Object】 This paper aims to explore the effect of applying high carbon-based soil remediation fertilizer on the bacterial community structure in the rhizosphere of flue-cured tobacco and the output value of tobacco leaves.【Method】 Using the field experiments method, two treatments were set up, including high carbon-based soil remediation fertilizer 0 kg·hm-2(CK) and 600 kg·hm-2(T), respectively.【Result】 The results showed that 90 days after tobacco transplanting, T treatment increased the content of soil organic matter, alkali hydrolyzable nitrogen, pH and water content by 23.59%, 17.41%, 1.07 unit and 24.17%, respectively; The soil sucrase activity, neutral phosphatase activity, urease activity and catalase activity by 97.65%, 363.64%, 356.60% and 19.02%, respectively. Meanwhile, T treatment significantly increased bacterial Chao index, Observed＿OTUs and Shannon index, and reduced the Simpson index. Among them, the relative abundance of GAL15, Gemmatimonadetes and Nitrospirae decreased significantly; The relative abundance of Thaumarchaeota increased significantly. In addition, the economic characters of tobacco leaves were significantly improved, reflecting in the increasement of top-grade tobacco proportion and output value by 12.74% and 12.15%, respectively. 【Conclusion】 The application of high carbon-based soil remediation fertilizer changed the soil bacterial community structure, increased soil nutrient content and enzyme activity, improved soil pH and the quality of tobacco leaves after curing.
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基本信息:
DOI：10.16445/j.cnki.1000-2340.20220825.001
中图分类号:S572;S154.3
引用信息:
[1]刘福童,李茂森,陈敏,等.高碳基土壤修复肥对烤烟根际土壤细菌群落结构及烟叶产值的影响[J],2022,56(05):724-731.DOI:10.16445/j.cnki.1000-2340.20220825.001.
基金信息:
国家重点研发计划(2021YFD1700900);; 陕西省烟草公司汉中市公司科技攻关项目(2021HZ05);; 安徽中烟工业有限责任公司科技攻关项目(20210331)