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【目的】在白花泡桐全基因组范围内鉴定细胞周期蛋白(cyclin,CYCs)基因家族成员，并研究其在泡桐丛枝病发生过程中的作用。【方法】利用生物信息学方法在白花泡桐全基因组范围内对PfCYCs家族成员进行鉴定，分析其系统发育关系、基因结构、理化性质和共线性。并对启动子顺式作用元件特征和蛋白互作进行预测。结合白花泡桐健康组培苗(Paulownia fortune,PF)和感染丛枝病组培苗(Paulownia fortunei infected with phytoplasmas,PFI)的转录组测序数据，分析PfCYCs基因在白花泡桐病健苗中的表达变化。【结果】白花泡桐CYCs基因家族共有48个成员，并在20条染色体上呈不均匀分布；系统发育分析将PfCYCs分为6种类型；共线性分析表明，32个PfCYCs存在片段复制现象。蛋白互作分析发现，A型和B型CYCs与WEE1等蛋白存在互作关系，D型CYCs与RBR1等蛋白存在互作关系。流式细胞仪检测发现感染泡桐丛枝病会改变细胞周期而影响细胞数量，特别是G2和S时期。转录组数据表明，22个PfCYCs基因在PFI中的表达上调，4个基因表达下调。【结论】在白花泡桐基因组中共鉴定出48个PfCYCs基因，其中PfCYCD3;1和PfCYCD5;1可能与丛枝病发生密切相关。
Abstract：【Objective】To identify cyclin(CYCs) gene family members across the whole genome of Paulownia fortunei(PF) and investigate their roles in the occurrence of paulownia witches' broom disease.【Method】Bioinformatics methods were employed to identify PfCYCs family members from the whole genome of Paulownia fortune, and to analyze their phylogenetic relationships, gene structures, physicochemical properties, and collinearity.The promoter cis-acting element features and proteinprotein interactions(PPI) were also predicted.Based on transcriptome sequencing data from Paulownia fortunei and Paulownia fortunei infected with phytoplasmas(PFI), the expression changes of PfCYCs genes in PF and PFI were analyzed.【Result】A total of 48 CYCs family members were identified in Paulownia fortunei, which were unevenly distributed across 20 chromosomes.Phylogenetic analysis classified PfCYCs into six types.Collinearity analysis revealed that 32 PfCYCs were involved in segmental duplication events.PPI analysis indicated that type A and B CYCs interact with proteins such as WEE1, while type D CYCs interact with proteins such as RBR1.Flow cytometry showed that Paulownia witches' broom disease alters the cell cycle and affects cell numbers, particularly in the G2 and S phases.Transcriptome analysis revealed that 22 PfCYCs genes were upregulated and 4 genes were downregulated in PFI.【Conclusion】In the genome of Paulownia fortunei, a total of 48 PfCYCs genes were identified, among which PfCYCD3;1 and PfCYCD5;1 are likely closely associated with the occurrence of witches' broom disease.
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基本信息:
DOI：10.16445/j.cnki.1000-2340.20250407.004
中图分类号:S792.43
引用信息:
[1]武文豪,李豫杰,范嘉铭,等.白花泡桐cyclin基因家族分析及对丛枝植原体的表达分析[J].河南农业大学学报,2026,60(02):237-250.DOI:10.16445/j.cnki.1000-2340.20250407.004.
基金信息:
中原科技创新领军人才项目（224200510010）
2025-01-12
2025
2025-03-27
2025-03-31
2025
1
2025-04-08
2025-04-08
2025-04-08