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【目的】在油茶基因组范围内鉴定油茶CKX基因家族成员，并研究其在抗病过程中对该基因家族表达模式的影响，为进一步研究油茶CKX基因参与调控响应逆境的分子机制奠定基础。【方法】通过油茶全基因组数据对油茶CKX(CoCKX)基因家族成员进行鉴定与分析，包括蛋白理化性质分析、系统进化树构建、基因结构分析、蛋白二级结构及三级结构分析、启动子顺式作用元件分析、共线性分析，并以炭疽菌对油茶叶片进行侵染，分析处理后12、24、48 d的油茶CKX基因家族的表达模式。【结果】CoCKX基因家族中共有7个成员，根据进化关系分为4个亚族；油茶共15条染色体，其中油茶CKX基因家族分布在5条染色体上；7个CoCKX基因家族成员均属于亲水性蛋白，主要定位于叶绿体中，且在结构上主要由α-螺旋和无规则卷曲组成；7个基因家族成员均有外显子和内含子，有2个成员无非编码区域（UTR），蛋白结构较为相似，基序一致；顺式作用元件表明，CoCKX基因家族启动子区域存在大量与光响应、水杨酸、茉莉酸甲酯响应及逆境响应等相关元件；共线性结果表明，CoCKX基因家族有1对串联重复基因；在油茶叶片抗病3个时期中7个基因表达量相较于健康植株有不同程度的上调，其中CoCKX1/2基因上调表达量较高，可较大程度地调控油茶在抗病过程中的响应机制。【结论】CoCKX基因家族成员功能上存在一定差异，基因结构在进化中有较高的保守性；在受到炭疽菌侵害过程中，CKX基因表达量上调，推测其具有调控叶片抵御炭疽菌的能力。
Abstract：Abstrat:【Objective】Identifying the Camellia oleifera CKX gene family member within the common Ca. oleifera genome and studying the effects on the expression pattern of this family of genes during disease resistance have laid a certain foundation for further research on the molecular, mechanisms involved in the regulation of Ca. oleifera CKX genes in response to adversity.【Method】The members of Ca. oleifera CKX gene family were identified and analyzed by the whole genome data of Ca. oleifera, including protein physicochemical property analysis, phylogenetic tree construction, gene structure analysis, protein secondary structure and tertiary structure analysis, promoter cis-acting element analysis, and covariance analysis. The leaves of Ca. oleifera were inoculated with anthrax, and the expression patterns of the Ca. oleifera CKX gene family were analyzed at 12, 24 and 48 d postinoculation.【Result】 The CoCKX gene family has seven members, which are divided into four subfamilies according to the evolutionary relationship. Ca. oleifera has a total of 15 chromosomes, among which the Ca. oleifera CKX gene family is distributed on five chromosomes. The seven CoCKX genes are hydrophilic proteins, which are mainly localized in chloroplasts and are structurally composed of α-helices and irregular convolutions. All seven members of the gene family have exons and introns, and there are two members with no UTRs. Their proteins are more structurally similar, with a consistent base sequence. cis-acting elements showed that there were a large number of elements related to light response, salicylic acid, methyl jasmonate response and adversity response in the promoter region of the CoCKX gene family. The covariance results showed that there was one pair of tandem duplicated genes in the CoCKX gene family. The expression of seven genes was up-regulated at the three periods during disease resistance in Ca. oleifera leaves to varying degrees compared with that of the healthy plants. Notably, the up-regulated expression of the CoCKX1/2 genes was higher, which might regulate the response mechanism of the oil tea in the process of disease resistance to a greater extent.【Conclusion】The CoCKX gene family is functionally distinct, and the gene structure as a whole is evolutionarily highly conserved. CKX gene expression is up-regulated during disease resistance, and it is hypothesized that it has the ability to regulate the ability of leaves to withstand disease.
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基本信息:
DOI：10.16445/j.cnki.1000-2340.20241121.002
中图分类号:S794.4
引用信息:
[1]乔新派,郝文乾,叶希琛,等.油茶CKX基因家族鉴定及表达分析[J].河南农业大学学报,2025,59(04):614-624.DOI:10.16445/j.cnki.1000-2340.20241121.002.
基金信息:
国家自然科学基金项目(32171835); 河南农业大学高层次人才专项支持基金项目(30501511); 国家林业和草原局管理干部学院重点项目(202308)