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【目的】探明赤霉病抗性鉴定所用镰刀菌菌株的致病力强弱，筛选出致病力强的优势菌株，以期为今后的小麦赤霉病抗性鉴定和遗传育种工作提供参考。【方法】利用国家和河南省小麦新品种区域试验赤霉病抗性鉴定所用的F0301、F0609、F0980、F1312、14AY1-2、14KF3-8、14LY9-2-4、14YY1-3、14ZK1-4等9个镰刀菌菌株，从菌株的菌丝生长速率、毒素类型、产毒量和接种后病小穗率等方面研究分析所用菌株的致病力强弱。【结果】9个菌株致病力强弱顺序为F1312、14AY1-2、14YY1-3 > F0301 > 14KF3-8 > 14ZK1-4、 14LY9-2-4 > F0980 > F0609。F1312、14AY1-2、14YY1-3处理的致病力差异不显著；国家区试菌株混合、河南区试菌株混合、14KF3-8、14LY9-2-4、14ZK1-4 5个处理的致病力差异不显著。【结论】F0609菌株致病力较弱，对小麦材料的赤霉病鉴定结果与前人鉴定结果相比表现较轻，在以后的抗赤霉病区域试验接种鉴定中要多加关注该菌株的侵染情况。14AY1-2、14YY1-3、F1312菌株的致病力较强，在以后的抗赤霉病遗传育种和品种布局中，需要重视14AY1-2、14YY1-3、F1312菌株的发生变化，对赤霉病发病情况进行监测和预警，及时采取防治措施，减少赤霉病危害，确保小麦产量和食品安全。
Abstract：【Objective】It is imperative to investigate the pathogenicity of principal Fusarium isolates employed for both inoculation procedures and resistance evaluation against Fusarium head blight(FHB) in national and Henan Province regional trials of new wheat cultivars.The selection of dominant isolates demonstrating robust pathogenicity aims to establish a reference system for future FHB resistance identification and genetic improvement in wheat breeding programs.【Method】Nine Fusarium isolates(F0301, F0609, F0980, F1312, 14 AY1-2, 14 KF3-8, 14 LY9-2-4, 14 YY1-3, 14 ZK1-4) from national and Henan provincial wheat variety regional trials were evaluated for their pathogenic potential against Fusarium head blight.Pathogenicity assessment incorporated quantitative analysis of mycelial expansion dynamics, mycotoxin chemotypes, deoxynivalenol production capacity, and post-inoculation disease incidence quantification.【Result】Pathogenicity hierarchy analysis revealed a virulence gradient among the nine Fusarium isolates: F1312,14 AY1-2,14 YY1-3 > F0301 > 14 KF3-8 > 14 ZK1-4,14 LY9-2-4 > F0980 > F0609.Statistical analysis demonstrated no significant divergence within the F1312,14 AY1-2,14 YY1-3 cluster, nor among the composite national isolates, Henan provincial isolates, 14 KF3-8, 14 LY9-2-4, and 14 ZK1-4.【Conclusion】The pathogenicity of the F0609 isolate was weak, and the identification results of FHB on wheat materials were milder than those of previous studies.Therefore, more attention should be paid to the infection of this isolate in future FHB-resistant regional test inoculation identification.The pathogenicity of 14 AY1-2, 14 YY1-3, and F1312 isolates was strong.Therefore, it is necessary to pay attention to changes in the 14 AY1-2, 14 YY1-3, and F1312 isolates in future genetic breeding for resistance to FHB.This work may aid in monitoring FHB incidence, help promote timely control measures to reduce the damage caused by FHB and ensure wheat yield and food safety.
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基本信息:
DOI：10.16445/j.cnki.1000-2340.20250416.003
中图分类号:S435.121.45
引用信息:
[1]刘胜利,董纯豪,沈庆花,等.小麦赤霉病病菌中不同菌株致病力分析[J].河南农业大学学报,2026,60(02):208-216.DOI:10.16445/j.cnki.1000-2340.20250416.003.
基金信息:
国家自然科学基金面上项目（32372093）; 国家重点研发计划（2023YFD1201005-3）; 科技创新2030-重大项目（2023ZD0402504）
2025-04-17
2025-04-17
2025-04-17