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以平菇菌丝为试材,研究不同发酵时间(2、4、6、8、10 d)、不同体积分数(20%、40%、60%、80%、100%)的玉米芯培养料水浸提液对平菇菌丝长势、生物量、细胞形态、代谢相关酶(三磷酸腺苷酶、琥珀酸脱氢酶、碱性磷酸酶)和胞外酶(羧甲基纤维素酶、木聚糖酶、漆酶、蛋白酶)活性的影响。结果表明:发酵10 d的培养料浸提液对平菇菌丝生长促进作用最为明显,尤其是体积分数为80%时,平菇菌丝生长速度和生物量分别比对照增加0.53 cm·d-1和1.79 mg,生长速度与100%体积分数无差异,生物量增加2.65 mg;三磷酸腺苷酶、琥珀酸脱氢酶、碱性磷酸酶活性分别比对照高2.6、6.4和4 825 U·g-1;漆酶、蛋白酶活性分别比对照高11.73、47.6 U·min-1·mL-1;此外,三磷酸腺苷酶、琥珀酸脱氢酶、碱性磷酸酶、漆酶、蛋白酶分别比100%体积分数时高5、7.2、2 201 U·g-1、2.0 U·min-1·mL-1及14.2 U·min-1·mL-1,羧甲基纤维素酶增加了13.09 U·min-1·mL-1。此外,透射电子显微镜观察发现,80%体积分数平菇菌丝细胞内线粒体及囊状体数量较对照明显增加。发酵培养料水浸提液能够明显促进平菇菌丝的生长。
Abstract：Taking the mycelium of Pleurotus ostreatus as testing material, the effects of aqueous extracts of corn cob medium with different fermentation time(2, 4, 6, 8 and 10 d)and volume fraction(20%, 40%, 60%, 80% and 100%)on the mycelial growth, biomass, cellular morphology, the activities of enzymes related to metabolism(adenosine triphosphatase, succinodehydrogenase, alkaline phosphatase) and extracellular enzymes(carboxymethyl cellulose enzyme, xylanase, laccase protease) were studied. Experimental results show that: the growth promotion of P.ostreatus mycelium was the most obvious after culturing 10 d with corn cob material extract, especially when the volume fraction was 80%. Compared with the control, the growth rate and biomass of P.ostreatus mycelia were increased by 0.53 cm·d-1and 1.79 mg, respectively, however, there was no difference between the growth rate and was 100% volume fraction. The biomass was increased by 2.65 mg; the activities of adenosine triphosphatase, succinodehydrogenase, alkaline phosphatase was 2.6, 6.4 and 4 825 U·g-1 higher than that of control; the laccase and protease activity was 11.73, 47.6 U·min-1·mL-1 higher than that of control, respectively; In addition, adenosine triphosphate, succinodehydrogenase, alkaline phosphatase, laccase, and protease was 5, 7.2, 2 201 U·g-1, 2.0 U·min-1·mL-1 and 14.2 U·min-1·mL-1 higher than 100% volume fraction, respectively, and carboxymethyl cellulase was increased by 13.09 U·min-1·mL-1. In addition, it was found that the number of mitochondria and cysts in the mycelia of P. ostreatus with 80% volume fraction was significantly increased compared with the control transmission electron microscopy. The water extract of fermentation medium could significantly promote the growth of P. ostreatus mycelia.
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基本信息:
DOI：10.16445/j.cnki.1000-2340.20210323.001
中图分类号:S646.14
引用信息:
[1]胡素娟,宋凯博,刘芹,等.玉米芯发酵料水浸提液促平菇菌丝生长的机理研究[J].河南农业大学学报,2021,55(02):273-280.DOI:10.16445/j.cnki.1000-2340.20210323.001.
基金信息:
河南省现代农业产业技术体系专项资金资助项目(S2013-09,S2013-09-G02); 河南省重大公益性行业专项(201300110700)
2021-03-24
2021-03-24
2021-03-24