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【目的】对白花泡桐全基因组同源异型盒（Homeobox， HB）基因家族成员进行鉴定，并研究该基因家族在白花泡桐丛枝病发生过程中的调控作用。【方法】通过生物信息学分析鉴定白花泡桐PfHBs基因家族成员，分析其理化性质、系统发育关系、种内种间共线性和启动子顺式作用元件等。利用String和AlphaFold3对互作蛋白质进行预测。结合白花泡桐植原体侵染前后的转录组测序数据，筛选出PfHBs家族中与丛枝病发生相关的成员。【结果】在白花泡桐中共鉴定出121个PfHBs基因家族成员，进化分析显示它们主要来源于片段重复和串联重复，可分为10个亚家族。基因表达水平分析和亚细胞定位结果表明，基因家族成员PfHB94基因在茎中高表达，编码蛋白定位于细胞核，并发现其可能与PaWB引发的早期免疫反应相关。蛋白质互作分析、分子对接与酵母双杂交试验证实，蛋白PfHB94与PfHB98存在相互作用。【结论】白花泡桐PfHBs家族共有121个成员，其中成员PfHB94与PfHB98通过互作可能调控SA合成相关基因表达，从而响应植原体侵染引发免疫反应。
Abstract：【Objective】To identify the members of the Homeobox (HB) gene family in the P. fortunei genome and investigate the role of this gene family during the development of Paulownia witches' broom (PaWB).【Method】Members of the PfHBs gene family were identified through bioinformatics analysis. Their physicochemical properties， phylogenetic relationships， intra- and interspecific collinearity， and promoter cis-acting elements were systematically analyzed. Protein-protein interactions were predicted using String and AlphaFold3. Furthermore， by integrating transcriptome sequencing data from P. fortunei before and after phytoplasma infection， members of the PfHBs family associated with witches' broom disease development were identified.【Result】A total of 121 PfHBs gene family members were identified in P. fortunei. Evolutionary analysis revealed that they primarily originated from segmental and tandem duplication events， and could be classified into 10 subfamilies. Gene expression analysis and subcellular localization analyses revealed that PfHB94， a member of the gene family that encodes a protein lacalized to the nucleus， is highly expressed in stems， may be associated with the early immune response triggered by PaWB. Protein-protein interaction analysis， molecular docking， and yeast two-hybrid experiments confirmed an interaction between PfHB94 and PfHB98.【Conclusion】Togeter ， our study indicated PfHB94 and PfHB98 likely regulates the expression of salicylic acid biosynthesis-related genes， thereby triggering an immune response against phytoplasma infection.
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基本信息:
DOI：10.16445/j.cnki.1000-2340.20260515.001
中图分类号:S792.43
引用信息:
[1]刘洁,吕钰洁,范国强,等.泡桐PfHBs基因家族鉴定及响应植原体侵染的免疫应答分析[J].河南农业大学学报().DOI:10.16445/j.cnki.1000-2340.20260515.001.
基金信息:
国家自然科学基金青年基金项目（32501689）; 国家资助博士后研究人员计划项目（B/C档，GZ20230711）; 河南省自然科学青年基金项目（25230042067）
2026-05-15
2026-05-15
2026-05-15